QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zambonino Infante, J. L. Articles by Cahu, C. L. Search for Related Content PubMed PubMed Citation Articles by Zambonino Infante, J. L. Articles by Cahu, C. L.

---

Nutrient Requirements

High Dietary Lipid Levels Enhance Digestive Tract Maturation and Improve Dicentrarchus labrax Larval Development

Unité Mixte de Nutrition des Poissons IFREMER-INRA, 29280 Plouzané, France

¹To whom correspondence should be addressed.

This study was designed to determine the nutritional lipid requirement of seabass larvae and to understand the effects of dietary fat concentration on their digestive tract maturation. Seabass (Dicentrarchus labrax) larvae were fed, from d 15 to 38 of life, one of five isonitrogenous compound diets with different lipid levels, ranging from 10 to 30 g/100 g. The higher the lipid level, the greater the growth and survival of the larvae (P < 0.05). The lipolytic enzymes assayed, lipase and phospholipase A₂, were stimulated by the increase in their respective dietary substrates, triglycerides and phospholipids, in 38-d-old larvae (P < 0.05). Nevertheless, a plateau in the activity of these two lipolytic enzymes was observed from 20% dietary lipids onwards. The similar mRNA levels of phospholipase A₂ in the three groups fed the highest lipid levels suggested that the maximal synthesis level of lipolytic enzyme was reached at 20% dietary fat. Pancreatic secretion of trypsin and amylase were positively affected by the dietary lipid level; a possible involvement of a cholecystokinin-releasing factor is discussed. Diets containing >20% lipids led to the increase in activities of brush border membrane enzymes to the detriment of a cytosolic enzyme in enterocytes, leucine-alanine (Leu-Ala) peptidase. This enzymatic change reveals the earlier maturation of enterocytes in larva groups fed high lipid levels.

KEY WORDS: • Dicentrarchus labrax • dietary lipid • pancreatic enzymes • intestinal enzymes

This article has been cited by other articles:

				E. H. Lim, T. J. Lam, and J. L. Ding Single-Cell Protein Diet of a Novel Recombinant Vitellogenin Yeast Enhances Growth and Survival of First-Feeding Tilapia (Oreochromis mossambicus) Larvae J. Nutr., March 1, 2005; 135(3): 513 - 518. [Abstract] [Full Text] [PDF]

				S. Fontagné, G. Corraze, and P. Bergot Tricaproin, Tricaprin and Trilaurin Are Utilized More Efficiently than Tricaprylin by Carp (Cyprinus carpio L.) Larvae J. Nutr., August 1, 2000; 130(8): 2009 - 2015. [Abstract] [Full Text]