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Research Communication

Soybean Isoflavones Reduce Experimental Metastasis in Mice^{1, ,2}

Donghua Li, John A. Yee, Michael H. McGuire^*, Patricia A. Murphy and Lin Yan³

Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, ^* Department of Surgery, Creighton University School of Medicine, Omaha, NE 68131 and Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011

³To whom correspondence should be addressed.

We investigated the effect of dietary supplementation with isoflavones on pulmonary metastasis of B16BL6 murine melanoma cells in C57BL/6 mice. Mice were fed a basal AIN-93G diet or the basal diet supplemented with the isoflavones genistein and daidzein at 113 µmol/kg, 225 µmol/kg, 450 µmol/kg, or 900 µmol/kg for 2 wk before and after the intravenous injection of 0.5 x 10⁵ melanoma cells. At necropsy, the number and size of tumors that formed in the lungs were determined. The number of mice that had >15 lung tumors was 17 in the control group, and 16, 15, 13, and 10 in the groups fed isoflavones at 113 µmol/kg, 225 µmol/kg, 450 µmol/kg and 900 µmol/kg, respectively. The latter two were significantly different from the control (P 0.05). The median number of tumors in the control group was 67, and those in the isoflavone-supplemented groups were 57, 33, 32, and 17, respectively. The last was significantly different from the control (P 0.05). Dietary supplementation with isoflavones at 225 µmol/kg, 450 µmol/kg, and 900 µmol/kg also significantly decreased tumor size (median cross-sectional area and volume) compared to the control values. We conclude that dietary supplementation with isoflavones reduces experimental metastasis of melanoma cells in mice.

KEY WORDS: • mice • genistein • daidzein • melanoma • metastasis

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