QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Drescher, K. Articles by Hagemeister, H. Search for Related Content PubMed PubMed Citation Articles by Drescher, K. Articles by Hagemeister, H.

---

Articles

Recovery of ¹⁵N-Lactoferrin Is Higher Than That of ¹⁵N-Casein in the Small Intestine of Suckling, But Not Adult Miniature Pigs^{1, ,2, ,3}

Karsten Drescher^*, Nils Roos^*,4, Maria Pfeuffer^*, Hans-Martin Seyfert, Jürgen Schrezenmeir^* and Hans Hagemeister

^* Federal Dairy Research Centre, Department of Physiology and Biochemistry of Nutrition, D-24121 Kiel, Germany, and Research Institute for Biology of Farm Animals, Division of Nutritional Physiology, D-18059 Rostock, Germany

⁴ To whom correspondence should be addressed.

Abstract

Performance of biological functions of lactoferrin in the small intestine requires at least some resistance to degradation. Therefore, we studied prececal digestibility of lactoferrin in comparison to casein both in suckling and adult miniature pigs, applying ¹⁵N-labeled proteins. In study 1, 43 piglets (10-d-old), deprived of food for 12 h received 10 mL of sow's milk supplemented with 120 mg of ¹⁵N-labeled protein (porcine or bovine lactoferrin or bovine casein). Piglets were anesthetized 150 min later, after which the small intestine was excised, cut into three sections, and chyme was collected. In study 2, nine food-deprived boars fitted with T-canulae at the terminal ileum were given two semisynthetic experimental meals (204 g) in a cross-over design, 2 wk apart. One contained 7.5% (g/100 g) ¹⁵N-labeled bovine casein, the other 1.25% ¹⁵N-labeled bovine lactoferrin. Both were adjusted to 15% total protein with nonlabeled casein. Ileal chyme was collected from the canula over 33 h postprandially. All diets contained the indigestible marker chromic oxide. ¹⁵N-digestibility of lactoferrin, both porcine (84.4 ± 3.2%) and bovine (82.3 ± 4.8%), was significantly lower than casein digestibility (97.6 ± 0.5%) in the distal small intestine of suckling piglets (P < 0.05). Based on immunoblotting after acrylamide electrophoresis, 4.5% of non- and partially digested lactoferrin was found in the last third of the small intestine of piglets. In adult miniature pigs there was no difference in ¹⁵N-digestibility of bovine lactoferrin compared to bovine casein (90.7 ± 1.9% vs. 93.9 ± 1.0%, P > 0.05). In suckling miniature pigs, the reduced digestibility of lactoferrin may provide the prerequisite for biological actions along the whole intestinal tract. The source of lactoferrin, porcine or bovine, made no difference in this respect.

KEY WORDS: • lactoferrin • ¹⁵N • prececal digestibility • miniature pigs • suckling, adults

This article has been cited by other articles:

				H. Kuwata, K. Yamauchi, S. Teraguchi, Y. Ushida, Y. Shimokawa, T. Toida, and H. Hayasawa Functional Fragments of Ingested Lactoferrin Are Resistant to Proteolytic Degradation in the Gastrointestinal Tract of Adult Rats J. Nutr., August 1, 2001; 131(8): 2121 - 2127. [Abstract] [Full Text] [PDF]