![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Arkansas Children's Nutrition Center, Arkansas Children's Hospital Research Institute and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR 72205
2To whom correspondence should be addressed.
Hepatic CYP3A and CYP2B enzymes were studied in male Sprague-Dawley rats derived from 5–7 litters fed diets in which the protein source was either casein or soy protein isolate. At age 65 d, rats were gavaged with corn oil (vehicle) or 50 mg/kg dexamethasone. Hepatic expression of CYP3A and CYP2B1 mRNA, apoprotein and associated monooxygenase activities were measured. Consumption of soy diets significantly increased monooxygenase activity toward the following: the CYP3A substrates erythromycin and ethylmorphine N-demethylase; corticosterone and testosterone 6ß-hydroxylase; and apoprotein and mRNA expression of CYP3A2 (P < 0.05). Dexamethasone significantly induced turnover of erythromycin and testosterone, expression of CYP3A apoprotein, and expression of CYP3A1 and CYP3A2 mRNA (P < 0.05). In addition, significant diet-inducer interactions were observed in the expression of CYP3A apoprotein and activities toward ethylmorphine, corticosterone and testosterone (P < 0.05). Significant diet-inducer interactions were also observed on CYP2B1-dependent pentoxyresorufin O-depentylase activity (P < 0.05). However, although dexamethasone significantly induced CYP2B1 expression at the apoprotein and mRNA level (P < 0.05), no significant diet effects were observed. These data suggest potential effects of soy consumption on the metabolism of a wide variety of CYP3A and CYP2B1 substrates, especially in situations involving coexposure to CYP inducers.
KEY WORDS: • soy protein isolate • cytochrome P450 • induction • rats
This article has been cited by other articles:
|
|
 |
|
  G. Cherala, B. H. Shapiro, and A. P. D'mello Effect of Perinatal Low Protein Diets on the Ontogeny of Select Hepatic Cytochrome P450 Enzymes and Cytochrome P450 Reductase in the Rat Drug Metab. Dispos., July 1, 2007; 35(7): 1057 - 1063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, O. Mezei, and N. F. Shay Human and Murine Hepatic Sterol-12-{alpha}-Hydroxylase and Other Xenobiotic Metabolism mRNA Are Upregulated by Soy Isoflavones J. Nutr., July 1, 2007; 137(7): 1705 - 1712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Seidel, S.-C. Hung, H Lynn Kan, and B Bhaskar Gollapudi Background Gene Expression in Rat Kidney: Influence of Strain, Gender, and Diet Toxicol. Sci., November 1, 2006; 94(1): 226 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Ronis, Y. Chen, J. Badeaux, E. Laurenzana, and T. M. Badger Soy Protein Isolate Induces CYP3A1 and CYP3A2 in Prepubertal Rats Experimental Biology and Medicine, January 1, 2006; 231(1): 60 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Ronis, Y. Chen, C.-H. Jo, P. Simpson, and T. M. Badger Diets Containing Soy Protein Isolate Increase Hepatic CYP3A Expression and Inducibility in Weanling Male Rats Exposed during Early Development J. Nutr., December 1, 2004; 134(12): 3270 - 3276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Mezei, C. N. Chou, K. J. Kennedy, C. Tovar-Palacio, and N. F. Shay Hepatic Cytochrome p450-2A and Phosphoribosylpyrophosphate Synthetase-Associated Protein mRNA Are Induced in Gerbils after Consumption of Isoflavone-Containing Protein J. Nutr., September 1, 2002; 132(9): 2538 - 2544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kano, F. Ishikawa, S. Matsubara, H. Kikuchi-Hayakawa, and Y. Shimakawa Soymilk Products Affect Ethanol Absorption and Metabolism in Rats during Acute and Chronic Ethanol Intake J. Nutr., February 1, 2002; 132(2): 238 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Rowlands, L. He, R. Hakkak, M. J. J. Ronis, and T. M. Badger Soy and Whey Proteins Downregulate DMBA-Induced Liver and Mammary Gland CYP1 Expression in Female Rats J. Nutr., December 1, 2001; 131(12): 3281 - 3287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. GERHOLD, M. LU, J. XU, C. AUSTIN, C. T. CASKEY, and T. RUSHMORE Monitoring expression of genes involved in drug metabolism and toxicology using DNA microarrays Physiol Genomics, April 27, 2001; 5(4): 161 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Ronis, J. C. Rowlands, R. Hakkak, and T. M. Badger Inducibility of Hepatic CYP1A Enzymes by 3-Methylcholanthrene and Isosafrole Differs in Male Rats Fed Diets Containing Casein, Soy Protein Isolate or Whey from Conception to Adulthood J. Nutr., April 1, 2001; 131(4): 1180 - 1188. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. C. Rowlands, H. Wang, R. Hakkak, M. J. J. Ronis, H. W. Strobel, and T. M. Badger Chronic Intragastric Infusion of Ethanol-Containing Diets Induces CYP3A9 While Decreasing CYP3A2 in Male Rats J. Pharmacol. Exp. Ther., November 1, 2000; 295(2): 747 - 752. [Abstract] [Full Text]
|
|
