![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Manuscript received 29 September 1997. Initial reviews completed 30 December 1997. Revision accepted 16 May 1998.
,, and
Laboratoire de Physiopathologie de la Nutrition, CNRS-ESA 7059, Université Paris 7/D. DIDEROT, 75251 Paris Cedex 05, France; * Département de Gynécologie, Hôpital Cochin, 75014 Paris, France;
and Station de Recherche Porcine, INRA, 35590 St. Gilles, France
Recent developments in dual-energy X-ray absorptiometry (DXA) have rendered feasible the determination of whole-body composition in small laboratory animals by directly measuring fat, fat-free and mineral bone masses. Our aim was to evaluate this technique by cross-calibrating the DXA method with the carcass chemical analysis in a heterogeneous population of nondiabetic Wistar and diabetic GK rats (21 animals were used for precision error and reproducibility determinations and 26 were used for accuracy studies). We report that this technique is optimized for weights >200 g. The respective CV for lean mass, fat mass and percentage of fat mass determined in short-term or transversal studies was 1.1 ± 0.1, 3.0 ± 1.3 and 3.1 ± 0.4% (mean ± SD) respectively. Further, this technique is valid for rats weighing from 130 to 200 g by using three successive scans. In longitudinal studies, daily calibrations significantly increased the percentage of fat mass CV to 6.6 ± 3.3%, but it was significantly decreased to 3.0 ± 2.7% by the use of triplicate scans. The accuracy for DXA was excellent in reference to the chemical extraction technique (r2 = 0.95 for percentage of fat mass, P < 0.0001), using an adjustment factor of 0.75 (limits of agreement between the two methods for percentage of fat mass = 
1.7-2.3%). Mimicry of longitudinal changes in body composition with intraperitoneal injections of saline solution demonstrated a satisfactory detection of body component changes (
2% of error for each final component analyzed, when increasing total lean mass by 11.8%). We conclude that DXA is appropriate for rat whole-body composition determination, allowing reliable long-term follow-up of individual animals for the first time.
in vivo technique.
The Journal of Nutrition Vol. 128 No. 9 September 1998,
pp. 1550-1554
Copyright ©1998 by the American Society for Nutritional Sciences
This article has been cited by other articles:
|
|
 |
|
  B. S Amorim, C. B Ueta, B. C G Freitas, R. J Nassif, C. H. d. A. Gouveia, M. A Christoffolete, A. S Moriscot, C. L. Lancelloti, F. Llimona, H. V. Barbeiro, et al. A TR{beta}-selective agonist confers resistance to diet-induced obesity J. Endocrinol., November 1, 2009; 203(2): 291 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. N. Horcajada, V. Habauzit, A. Trzeciakiewicz, C. Morand, A. Gil-Izquierdo, J. Mardon, P. Lebecque, M. J. Davicco, W. S. S. Chee, V. Coxam, et al. Hesperidin inhibits ovariectomized-induced osteopenia and shows differential effects on bone mass and strength in young and adult intact rats J Appl Physiol, March 1, 2008; 104(3): 648 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Movassat, D. Bailbe, C. Lubrano-Berthelier, F. Picarel-Blanchot, E. Bertin, J. Mourot, and B. Portha Follow-up of GK rats during prediabetes highlights increased insulin action and fat deposition despite low insulin secretion Am J Physiol Endocrinol Metab, January 1, 2008; 294(1): E168 - E175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M Villicev, F. R S Freitas, M. S Aoki, C. Taffarel, T. S Scanlan, A. S Moriscot, M. O Ribeiro, A. C Bianco, and C. H A Gouveia Thyroid hormone receptor {beta}-specific agonist GC-1 increases energy expenditure and prevents fat-mass accumulation in rats J. Endocrinol., April 1, 2007; 193(1): 21 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. L. Burr, C. G. Taylor, and H. A. Weiler Dietary Conjugated Linoleic Acid Does Not Adversely Affect Bone Mass in Obese fa/fa or Lean Zucker Rats. Experimental Biology and Medicine, November 1, 2006; 231(10): 1602 - 1609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zaidi, K. A. James, and G. F. Wagner Passive immunization of lactating mice with stanniocalcin-1 antiserum reduces mammary gland development, milk fat content, and postnatal pup growth Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E974 - E981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Nelson and G. A. Iwamoto Reversibility of exercise-induced dendritic attenuation in brain cardiorespiratory and locomotor areas following exercise detraining J Appl Physiol, October 1, 2006; 101(4): 1243 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Nelson, J. M. Juraska, T. I. Musch, and G. A. Iwamoto Neuroplastic adaptations to exercise: neuronal remodeling in cardiorespiratory and locomotor areas J Appl Physiol, December 1, 2005; 99(6): 2312 - 2322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D Sainz and A. Urlando Evaluation of a new pediatric air-displacement plethysmograph for body-composition assessment by means of chemical analysis of bovine tissue phantoms Am. J. Clinical Nutrition, February 1, 2003; 77(2): 364 - 370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Cavalie, G. Lac, P. Lebecque, B. Chanteranne, M.-J. Davicco, and J.-P. Barlet Influence of clenbuterol on bone metabolism in exercised or sedentary rats J Appl Physiol, December 1, 2002; 93(6): 2034 - 2037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Peyron-Caso, M. Taverna, M. Guerre-Millo, A. Veronese, N. Pacher, G. Slama, and S. W. Rizkalla Dietary (n-3) Polyunsaturated Fatty Acids Up-Regulate Plasma Leptin in Insulin-Resistant Rats J. Nutr., August 1, 2002; 132(8): 2235 - 2240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Mei, L. M Grummer-Strawn, A. Pietrobelli, A. Goulding, M. I Goran, and W. H Dietz Validity of body mass index compared with other body-composition screening indexes for the assessment of body fatness in children and adolescents Am. J. Clinical Nutrition, June 1, 2002; 75(6): 978 - 985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sjogren, N. Hellberg, M. Bohlooly-Y, L. Savendahl, M. S. Johansson, T. Berglindh, I. Bosaeus, and C. Ohlsson Body Fat Content Can Be Predicted In Vivo in Mice Using a Modified Dual-Energy X-Ray Absorptiometry Technique J. Nutr., November 1, 2001; 131(11): 2963 - 2966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Levy, J. Lesko, R. J. Krieg Jr., R. A. Adler, and W. Stevens Leptin responses to glucose infusions in obesity-prone rats Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1088 - E1096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. McMinn, C. W. Wilkinson, P. J. Havel, S. C. Woods, and M. W. Schwartz Effect of intracerebroventricular alpha -MSH on food intake, adiposity, c-Fos induction, and neuropeptide expression Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2000; 279(2): R695 - R703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Veyrat-Durebex, P. Gaudreau, V. Coxam, N. Gaumet, and J. Alliot Peripheral injection of growth hormone stimulates protein intake in aged male and female Lou rats Am J Physiol Endocrinol Metab, June 1, 1999; 276(6): E1105 - E1111. [Abstract] [Full Text] [PDF]
|
|
