Food Deprivation Changes Peroxisomal -Oxidation Activity but Not Catalase Activity during Postnatal Development in Pig Tissues

Manuscript received 8 September 1997. Initial reviews completed 12 October 1997. Revision accepted 19 March 1998.

Xing Xian Yu^*, , James K. Drackley^{*, ,} , and Jack Odle^{*, ,}

^* Division of Nutritional Sciences and  Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801

Peroxisomal -oxidation and catalase activity were investigated in liver, kidney and heart from pigs at the following timepoints: within 0.5 h after birth (0 h, unfed) and at 24 h (suckled or unsuckled), 10 d (suckled or 24-h food-deprived), 21 d (suckled or 24-h food-deprived) and 5 mo (overnight food-deprived). In liver, peroxisomal -oxidation increased about twofold at 24 h for suckled pigs (P < 0.001) but did not change for unsuckled pigs. The rate was further increased in 21-d-old pigs compared with 0- (P < 0.001) or 24-h-old (P < 0.05) pigs, but was lower at 5 mo than at 10 or 21 d (P < 0.01). The rate was higher for food-deprived pigs than suckled pigs at 10 d (P < 0.001) of age. In kidney, peroxisomal -oxidation was unchanged during the first 24 h but was higher (P < 0.05) at 10 d for suckled pigs and at 21 d than at 0 h. Nutritional state did not influence renal peroxisomal -oxidation. In heart, peroxisomal -oxidation did not change with age or nutritional state. The developmental pattern of fatty acyl-CoA oxidase activity was similar to that of peroxisomal -oxidation in each tissue. Developmental increases of peroxisomal -oxidation were greater than those for first-cycle peroxisomal -oxidation reported earlier, suggesting that peroxisomal -oxidation became more complete in older pigs. Catalase activity did not change during the first 24 h after birth but then increased 10.5-, 2.9-fold and 33% at 10 d in liver, kidney and heart, respectively. The concentration of catalase mRNA was only 1.1- and 1.3-fold higher at 10 d than at 24 h in liver and kidney, respectively. Catalase activity was not affected by food deprivation. We concluded the following: 1) peroxisomal -oxidation develops rapidly after birth and may be important for piglets to oxidize milk fatty acids; 2) food is required for the initial induction after birth; and 3) rapidly increased catalase activity during the first 10 d of life resulted from both pretranslational and post-translational regulation.

The Journal of Nutrition Vol. 128 No. 7 July 1998, pp. 1114-1121
Copyright ©1998 by the American Society for Nutritional Sciences

This article has been cited by other articles:

				X. X. Yu, J. Odle, and J. K. Drackley Differential induction of peroxisomal beta -oxidation enzymes by clofibric acid and aspirin in piglet tissues Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2001; 281(5): R1553 - R1561. [Abstract] [Full Text] [PDF]