Lipogenic Enzyme Gene Expression Is Quickly Suppressed in Rats by a Small Amount of Exogenous Polyunsaturated Fatty Acids

Manuscript received 12 September 1997. Initial reviews completed 11 November 1997. Revision accepted 11 February 1998.

Tezukayama Gakuin College, Sakai, Osaka 590-01, Japan

An examination was conducted of the time courses of incorporation of polyunsaturated fatty acids (PUFA) into lipids of plasma, liver and its nuclei, and the time courses of hepatic lipogenic enzyme gene expression after oral administration of perilla oil by a stomach tube to rats fed a fat-free diet. Linolenic acid, 18:3(n-3), and eicosapentaenoic acid, 20:5(n-3), were considered indices of exogenous fatty acids. In total lipids of liver and its nuclei, linolenic acid was detected 1 h after the intubation, continued to increase during the first 4 h, then decreased and almost disappeared by 48 h. Eicosapentaenoic acid also increased within only 1 h of intubation, reached a maximum after 8 h and then gradually decreased. In contrast with the increase of exogenous PUFA, the mRNA concentrations of hepatic lipogenic enzymes began to decrease 2 h after the perilla oil intubation, were at a minimum at 8 h, and then increased. In another experiment to examine the effects of dietary perilla oil concentration on PUFA incorporation and gene expression, rats were given diets containing 0-10% perilla oil (supplemented with hydrogenated fat to 10% fat) for 3 d. Only 1% perilla oil elevated the exogenous PUFA concentrations in liver and its nuclei in comparison with concentrations in rats fed a hydrogenated fat diet. Perilla oil at 2% of the diet was sufficient to suppress lipogenic enzyme gene expressions, which were suppressed to the minimum level by 5% perilla oil in the diet. Thus, lipogenic enzyme gene expression was quickly suppressed by a small amount of exogenous PUFA, in contrast with the increase of PUFA incorporation into liver and its nuclei. Newly incorporated exogenous PUFA appear to be involved in suppression of lipogenic enzyme gene expression.

The Journal of Nutrition Vol. 128 No. 6 June 1998, pp. 967-972
Copyright ©1998 by the American Society for Nutritional Sciences

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