Ferrets (Mustela putoius furo) Inefficiently Convert -Carotene to Vitamin A

Manuscript received 21 April 1997. Initial reviews completed 19 June 1997. Revision accepted 20 October 1997.

Department of Food Science and Human Nutrition and Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801

The ferret has recently been used as a model to evaluate the absorption and metabolism of several carotenoids; however, little is known about the vitamin A (VA) requirements of this species or the ability of ferrets to convert dietary -carotene (C) to VA. Three studies were conducted to estimate the daily utilization of VA in ferrets and to determine the effect of prior VA status on the ability of ferrets to utilize C as a source of VA. Weanling male ferrets were fed a pelleted, low carotenoid, semipurified diet either with (+VA) or without VA (VA) for 21- to 35-d prefeeding periods. Upon initiation of the experiments, several ferrets were killed to determine base-line VA status. The remaining ferrets were fed VA, C, or VA and C in pelleted feed (Studies 1-3) or liquid carrier (Study 3) for 16-21 additional days. Hepatic VA and C concentrations were used as the primary indicators of VA status, although serum and adrenal VA and C also were measured. The results showed the following: 1) provision of C at up to a 15:1 weight ratio of C to VA in pelleted feed or liquid carrier was not sufficient to maintain hepatic VA stores after a VA prefeeding period; 2) the daily utilization rate of VA by ferrets ranged from 80 to 171 µg in the three studies; 3) the ferret was confirmed to be a species that has the majority of its serum VA in ester form; and 4) feeding VA diets significantly reduced serum retinyl esters but had less effect on serum retinol. We conclude that although ferrets can convert C to VA, the process is inefficient. The ferret model can be most appropriately used when studying the biological effect of tissue C stores on VA status and is less appropriate for the evaluation of dietary C conversion to VA.

The Journal of Nutrition Vol. 128 No. 2 February 1998, pp. 271-279
Copyright ©1998 by the American Society for Nutritional Sciences

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