Natural Dietary Polyphenolic Compounds Cause Endothelium-Dependent Vasorelaxation in Rat Thoracic Aorta

Manuscript received 8 May 1998. Initial reviews completed 1 July 1998. Revision accepted 17 August 1998.

Emile Andriambeloson^*, Céline Magnier^*, Gisèle Haan-Archipoff, Annelise Lobstein, Robert Anton, Alain Beretz^*, Jean Claude Stoclet^*, and Ramaroson Andriantsitohaina^*,

^* Laboratoire de Pharmacologie et Physiopathologie Cellulaires, Université Louis Pasteur de Strasbourg, CNRS ERS 653 Faculté de Pharmacie, BP 24, 67401 Illkirch-Cedex, France and  Laboratoire de Pharmacognosie, Université Louis Pasteur de Strasbourg, CNRS GDR 1206 Faculté de Pharmacie, BP 24, 67401 Illkirch-Cedex, France

This study investigated the possible active principles which support the endothelial nitric oxide-dependent relaxation produced by red wine and other plant polyphenolic compounds in thoracic aorta from male Wistar rats (12-14 wk old). Relaxation experiments were recorded isometrically on vessels precontracted with norepinephrine. Ten different chromatographic fractions (3-18 mg) isolated from red wine polyphenolic compounds (RWPC) and some available defined polyphenols (10-15 mg) were tested. Fractions enriched into either anthocyanins or oligomeric condensed tannins exhibited endothelium-dependent vasorelaxant activity (maximal relaxation in the range of 59-77%) comparable to the original RWPC. However, polymeric condensed tannins elicited a weaker vasorelaxant activity than the original RWPC (maximal relaxation ranged between 20-47%, P < 0.01). Moreover, the representative of either phenolic acid derivatives (benzoic acid, vanillic acid, gallic acid), hydroxycinnamic acid (p-coumaric acid, caffeic acid) or the flavanol [(+)-epicatechin] classes failed to induce this type of response. Among the anthocyanins, delphinidin (maximal relaxation being 89%), but not malvidin or cyanidin, showed endothelium-dependent vasorelaxation. These results show that anthocyanins and oligomeric-condensed tannins exhibited a pharmacological profile comparable to the original RWPC. These compounds may be involved in the reduction of cardiovascular mortality related to the presence of wine, fruits and vegetables in the diet.

The Journal of Nutrition Vol. 128 No. 12 December 1998, pp. 2324-2333
Copyright ©1998 by the American Society for Nutritional Sciences

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