Dietary Amylose-Amylopectin Starch Content Affects Glucose and Lipid Metabolism in Adipocytes of Normal and Diabetic Rats

Manuscript received 5 December 1996. Initial reviews completed 5 March 1997. Revision accepted 3 September 1997.

Department of Diabetes, INSERM U341, University of Pierre et Marie Curie, Hôtel-Dieu Hospital, 75004 Paris, France and ^* Laboratory of Nutrition INRA, 44026 Nantes, France

The aim of this study was to evaluate the effects of the chronic consumption of two starches, characterized by different glycemic indices and amylose-amylopectin content, on glucose metabolism in rat epididymal adipocytes. The two chosen starches were from mung bean (32% amylose) and cornstarch (0.5% amylose). The -amylase digestibility was higher for the waxy cornstarch than that of the mung bean starch (60 ± 4 vs. 45 ± 3%, mean ± SEM, respectively). The glycemic index of the waxy cornstarch diet (575 g starch /kg diet) was higher than that of the mung bean starch diet (107 ± 7 vs. 67 ± 5%, P < 0.01) when measured in vivo in two groups of normal rats (n = 9). In a subsequent study, normal and diabetic (streptozotocin-injected on d 2 of life) male Sprague-Dawley rats (18 per group) consumed a diet containing 575 g starch/kg diet as either waxy cornstarch or mung bean starch. After 3 wk, food intake, epididymal fat pad weights, and plasma glucose, insulin and triglyceride concentrations did not differ between diet groups. Adipocyte diameter was smaller in rats that consumed mung bean starch compared with those that consumed the waxy cornstarch diet (P < 0.01). The mung bean diet increased maximal insulin-stimulated ¹⁴C-glucose oxidation (% of basal values, P < 0.05). In contrast, incorporation of ¹⁴C-glucose into total lipids was significantly lower in rats that consumed the mung bean diet (P < 0.05). We conclude that in both normal and diabetic rats, the chronic replacement of a high glycemic index starch by a low glycemic index one in a mixed diet increases insulin-stimulated glucose oxidation, decreases glucose incorporation into total lipids and decreases epididymal adipocyte diameter. Thus, the type of starch mixed into the diet has important metabolic consequences at the cellular level in both normal and diabetic rats.

The Journal of Nutrition Vol. 128 No. 1 January 1998, pp. 35-43
Copyright ©1998 by the American Society for Nutritional Sciences

This article has been cited by other articles:

				E. M. van Schothorst, A. Bunschoten, P. Schrauwen, R. P. Mensink, and J. Keijer Effects of a high-fat, low- versus high-glycemic index diet: retardation of insulin resistance involves adipose tissue modulation FASEB J, April 1, 2009; 23(4): 1092 - 1101. [Abstract] [Full Text] [PDF]

				K. B. Scribner, D. B. Pawlak, C. M. Aubin, J. A. Majzoub, and D. S. Ludwig Long-term effects of dietary glycemic index on adiposity, energy metabolism, and physical activity in mice Am J Physiol Endocrinol Metab, November 1, 2008; 295(5): E1126 - E1131. [Abstract] [Full Text] [PDF]

				Y. Ma, B. Olendzki, D. Chiriboga, J. R. Hebert, Y. Li, W. Li, M. Campbell, K. Gendreau, and I. S. Ockene Association between Dietary Carbohydrates and Body Weight Am. J. Epidemiol., February 15, 2005; 161(4): 359 - 367. [Abstract] [Full Text] [PDF]

				J. C Brand-Miller, S. H. Holt, D. B Pawlak, and J. McMillan Glycemic index and obesity Am. J. Clinical Nutrition, July 1, 2002; 76(1): 281S - 285. [Abstract] [Full Text] [PDF]

				D. S. Ludwig The Glycemic Index: Physiological Mechanisms Relating to Obesity, Diabetes, and Cardiovascular Disease JAMA, May 8, 2002; 287(18): 2414 - 2423. [Abstract] [Full Text] [PDF]

				C. Bouche, S. W. Rizkalla, J. Luo, H. Vidal, A. Veronese, N. Pacher, C. Fouquet, V. Lang, and G. Slama Five-Week, Low-Glycemic Index Diet Decreases Total Fat Mass and Improves Plasma Lipid Profile in Moderately Overweight Nondiabetic Men Diabetes Care, May 1, 2002; 25(5): 822 - 828. [Abstract] [Full Text] [PDF]

				L. E. Spieth, J. D. Harnish, C. M. Lenders, L. B. Raezer, M. A. Pereira, S. J. Hangen, and D. S. Ludwig A Low-Glycemic Index Diet in the Treatment of Pediatric Obesity Arch Pediatr Adolesc Med, September 1, 2000; 154(9): 947 - 951. [Abstract] [Full Text] [PDF]

				V. Lang, P. Vaugelade, F. Bernard, B. Darcy-Vrillon, C. Alamowitch, G. Slama, P.-H. Duee, and F. R. Bornet Euglycemic hyperinsulinemic clamp to assess posthepatic glucose appearance after carbohydrate loading. 1. Validation in pigs Am. J. Clinical Nutrition, June 1, 1999; 69(6): 1174 - 1182. [Abstract] [Full Text] [PDF]

				V. Lang, F. R. Bornet, P. Vaugelade, M. van Ypersele de Strihou, J. Luo, N. Pacher, F. Rossi, P. La Droitte, P.-H. Duee, and G. Slama Euglycemic hyperinsulinemic clamp to assess posthepatic glucose appearance after carbohydrate loading. 2. Evaluation of corn and mung bean starches in healthy men Am. J. Clinical Nutrition, June 1, 1999; 69(6): 1183 - 1188. [Abstract] [Full Text] [PDF]

				M. Kabir, S. W. Rizkalla, A. Quignard-Boulangé, M. Guerre-Millo, J. Boillot, B. Ardouin, J. Luo, and G. Slama A High Glycemic Index Starch Diet Affects Lipid Storage-Related Enzymes in Normal and to a Lesser Extent in Diabetic Rats J. Nutr., November 1, 1998; 128(11): 1878 - 1883. [Abstract] [Full Text]