The Journal of Nutrition Vol. 127 No. 8 August 1997, pp. 1636S-1645S
Copyright ©1997 by the American Society for Nutritional Sciences

Olestra Affects Serum Concentrations of -Tocopherol and Carotenoids but not Vitamin D or Vitamin K Status in Free-Living Subjects

The Procter & Gamble Company, Cincinnati, OH, 45224 and ^* The Department of Family Medicine, Indiana University School of Medicine, Indianapolis, IN 46256

Normal, healthy, free-living adults ingested either 18 g/d olestra, with or without 1.1 mg tocopheryl acetate/g olestra, or 18 g/d triglyceride placebo, for 16 wk in a double-blind, placebo-controlled study. Serum concentrations of -tocopherol, -carotene, -carotene, lycopene, lutein/zeaxanthin, retinol and cholesterol were measured biweekly. Serum 25-hydroxyvitamin D concentration, prothrombin time, partial thromboplastin time and plasma concentration of functional prothrombin (Simplastin-Ecarin assay) were measured at wk 0, 8 and 16. Relative to the placebo group, serum -tocopherol concentration was reduced 6% for the group given 18 g/d olestra. Addition of tocopheryl acetate to olestra partially offset the effect of olestra. For the group given 18 g/d olestra plus 1.1 mg tocopheryl acetate/g olestra, serum -tocopherol concentration was 4% less than the placebo value. Olestra reduced serum concentration of -carotene by 27%; the other carotenoids were similarly affected. Serum cholesterol concentration was reduced ~4.5% in the olestra groups, relative to placebo, but the differences were not significant. Serum triglycerides, serum 25-hydroxyvitamin D, prothrombin time, partial thromboplastin time or the plasma concentration of under--carboxylated prothrombin were unaffected by olestra. Clinical observations and laboratory measures indicated no health-related effects of olestra; mild-to-moderate transient gastrointestinal symptoms such as bloating, cramping, loose stools and diarrhea were reported by all groups.

Key words: -carotene, carotenoids, olestra, vitamin E, vitamin K, vitamin D, humans.