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Manuscript received 21 February 1996. Initial reviews completed 21 May 1996. Revision accepted 8 October 1996.
Gastrointestinal Research Center, Division of Gastroenterology, Department of Medicine, University Hospital Gasthuisberg, Katholieke Universiteit Leuven, 3000 Leuven, Belgium and * Laboratory of Physiology and Immunology of Domestic Animals, Faculty of Agricultural and Applied Biological Sciences, Department of Animal Production, 3001 Heverlee, Belgium
Protein assimilation and metabolism studies are hindered by the lack of an adequate oral tracer, i.e., labeled proteins. We present a new and easily reproducible methodology for producing large amounts of egg proteins labeled with L-leucine-13C1. Laying hens were fed a 0.2% leucine-deficient food supplemented with 0.2% L-leucine-13C1 (99 atom %). At plateau, eggs containing highly enriched proteins were obtained. The 13C content of egg white relative to the total C content was 1.3371 atom %, corresponding to 
= 206
. The overall tracer recovery in egg proteins was high (40.2%), making this method financially attractive as well. Accurately measurable levels of 13CO2 in breath were obtained after ingestion of a physiological load of labeled egg white proteins. Thus, egg proteins with sufficient 13C enrichment and applicable for human protein assimilation and metabolism kinetic studies were produced in an easily reproducible and highly efficient manner.
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