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A Nucleoside-Nucleotide Mixture and Its Components Increase Lymphoproliferative and Delayed Hypersensitivity Responses in Mice¹

Keiko Yamauchi, Andrew A. Adjei^*, Clement K. Ameho, Yin-Ching Chan^**, Anil D. Kulkarni, Seiji Sato, Keiichi Okamoto and Shigeru Yamamoto²

Department of Nutrition ^* Department of Bacteriology ^** Research Center of Comprehensive Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa 903-01 Department of Surgery, St. Louis Medical Center, St. Louis, MO Ohtsuka Pharmaceutical Co., Naruto 772, Japan

We studied the effect of individual components of a nucleoside-nucleotide mixture on T cell-mediated immunity in BALB/c and DBA/2 mice. Mice were fed for 4 wk a nucleotide-free 20% casein diet (control) or this diet supplemented with 3 mol/kg of one of the following; inosine, guanosine monophosphate, uridine, cytidine, thymidine, or a mixture of these. In both strains of mice, popliteal lymph node immunoproliferative response to alloantigeneic (C57BL/6 splenic cells) challenge in mice fed the mixture and guanosine monophosphate was greater (P < 0.05) than in the mice fed the control diet. BALB/c mice fed inosine, uridine, and thymidine also showed greater (P < 0.05) responses compared with control fed mice. In the sheep red blood cells challenge assay, foot pad weight-gain in both strains of mice fed the mixture and the individual components supplemented diets was greater (P < 0.05) than in those fed the control diet. In both strains of mice, interleukin-2 secretion by popliteal lymph node lymphocytes in mice fed the mixture and thymidine was higher (P < 0.05) compared with the rest of the groups except BALB/c mice fed cytidine. Significantly higher than control secretions of interferon- were observed only in BALB/c mice fed inosine, thymidine and the mixture. We conclude that mice fed the nucleoside-nucleotide mixture and the individual components of the mixture had greater responses in the T cell-mediated immune functions studied and that the responses in mice fed the mixture were not different from those in mice fed the individual components.

KEY WORDS: • nucleosides • nucleotides • delayed-type hypersensitivity • cytokines • mice

¹ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

² To whom correspondence should be addressed.

Manuscript received 12 June 1995. Revision accepted 29 February 1996.

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