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Dietary Rosemary Suppresses 7,12-Dimethylbenz(a)anthracene Binding to Rat Mammary Cell DNA^1,2,

Department of Nutrition, The Pennsylvania State University, University Park, PA 16802

Commercially available ground rosemary powder was examined for its ability to modify the in vivo binding of 7,12-dimethylbenz(a)anthracene (DMBA) metabolites to mammary cell DNA in 55-d-old rats fed diets containing varying quantities and types of lipids. Supplementing a casein-based diet containing 20% corn oil with 1% rosemary for 2 wk reduced by 76% the occurrence of DMBA-induced DNA adducts occurring 24 h after treatment with 50 mg DMBA/kg body weight. A comparable reduction in DNA adducts (66%) occurred when 0.5% rosemary was added to a diet containing 20% corn oil, and the quantity of DMBA given was reduced to 25 mg/kg body weight. The reduction in the occurrence of adducts occurring 24 h after DMBA treatment caused by 0.5% dietary rosemary was greater (P < 0.05) when added to a diet containing 20% corn oil than when added to a diet containing 5% corn oil and 15% coconut oil. Rosemary, 1% but not 0.5%, reduced DMBA-induced DNA adducts when the diet contained 5% corn oil. These studies demonstrate that rosemary is effective in reducing the binding of DMBA metabolites to rat mammary cell DNA. Furthermore, the present studies demonstrate that the benefits of rosemary are dependent on the source and concentration of dietary lipids.

KEY WORDS: • 7,12-dimethylbenz(a)anthracene • DNA adducts • lipid • rats • rosemary

¹ Supported in part by a grant from the American Institute for Cancer Research (Grant 223K) and Wakunaga of America, Mission Viejo, CA 9261.

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ Present Address: Wakunaga of America Co., Ltd., 23501 Madero, Mission Viego, CA 92691.

⁴ Present address: 121 Buckhorn Rd., Richboro, PA 18954.

⁵ To whom reprint requests should be adressed.

Manuscript received 15 August 1994. Revision accepted 9 January 1996.

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