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Intracellular ß-Carotene Transport in Bovine Liver and Intestine Is Not Mediated by Cytosolic Proteins^1,2,

Eric T. Gugger^* and John W. Erdman, Jr.^*,,3

^* Department of Food Science Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801

The mechanisms responsible for the movement of absorbed carotenoids between intracellular organelles and for the incorporation of carotenoids into serum lipoproteins are unknown. It was the objective of this study to use bovine liver and intestine as a model to study the possible cytosolic protein-mediated carotenoid transfer between liposomes and mitochondria in vitro. Liposomes containing ß-[³H]carotene were incubated with isolated bovine hepatic mitochondria (1 mg protein) and various quantities of liver cytosol or intestinal mucosal cell cytosol (0–6 mg protein) for up to 1 h at 37°C. The ß-[³H]carotene activity in liposomes was then measured over time to determine percent transfer of ß-carotene to mitochondria. Over the time period studied, the rate of ß-carotene transfer was unaffected by increasing levels of either hepatic or intestinal cytosolic protein. These results suggest that unlike many other lipid species, intracellular transport of ß-carotene is not mediated by cytosolic transport proteins and must occur by other mechanisms such as vesicular transport or by membrane-bound proteins.

KEY WORDS: • ß-carotene • transport • bovine • liver • intestine

¹ Supported by NRI/USDA competitive grants program agreement No. 91-37200-6273.

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ To whom reprint requests should be addressed at Division of Nutritional Sciences, University of Illinois, 905 S. Goodwin, 451 Bevier Hall, Urbana, IL 61801.

Manuscript received 10 August 1995. Revision accepted 6 February 1996.