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Kinetic Model of Molybdenum Metabolism Develped from Dual Stable Isotope Excretion in Men Consuming a Low Molybdenum Diet^1,2,3,

Western Human Nutrition Research Center, U.S. Department of Agriculture, Agricultural Research Service, Presidio of San Francisco, CA 94129-0997

The aim of this study was to develop a compartmental model of molybdenum metabolism based on stable isotope excretion patterns. Molybdenum (Mo) is an essential trace element in humans, with an estimated safe and adequate daily dietary intake (ESADDI) of 75–750 µg Mo/d. Four adult men were fed low molybdenum diets, 22 µg Mo/d, for a period of 102 d. ⁹⁷Mo and ¹⁰⁰Mo stable isotopes, in intravenous and oral doses, respectively, were administered at selected intervals. The resulting 6-d cumulative urinary and fecal isotope excreation data were used to model molybdenum metabolism using SAAM/CONSAM software, A kinetic model, including gastrointestinal (GI), plasma, slow-turnover tissue and fast-turnover tissue compartments, accurately simulated the observed pattern of urinary and fecal excretion for both stable isotopes in all four subjects. Residence time for molybdenum in the GI tract was estimated at 1.7 ± 0.4 d. Predicted residence time for plasma molybdenum was 22 ± 4 min, whereas slow-turnover tissue (possibly hepatic) retention averaged 58 ± 16 d. The model thus permitted estimation of kinetic parameters for molybdenum metabolism in tissues not readily accessible of measurable in humans.

KEY WORDS: • molybdenum • compartmental • analysis • SAAM/CONSAM • humans • kinetics

¹ Portions of this work were presented at Experimental Biology '95, April 9–13, 1995, in Atlanta, GA. [Thompson, K.H. & Turnlund, J. R. (1995) Compartmental model of molybdenum metabolism in adult men fed a low molybdenum diet. FASEB J. 9: A1000 (abs.)].

² Product names are necessary to report factually on methods used; however, the U.S. Department of Agriculture neither guarantees nor warrants the standard of the product, and the use of a product name by the USDA implies no approval of the product to the exclusion of others that may be suitable.

³ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

⁴ To whom reprint requests should be addressed.

Manuscript received 31 July 1995. Revision accepted 4 January 1996.

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