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Methionine and 2-Hydroxy-4-Methylthiobutanoic Acid Are Transported by Distinct Na⁺-Dependent and H⁺-Dependent Systems in the Brush Border Membrane of the Chick Intestinal Epithelium^1,2,3,

Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5B5

The pathways that facilitate the uptake of L-methionine (L-Met), D-methionine (D-Met) and L-2-hydroxy-4-methylthiobutanoic acid (L-HMB) were characterized in chick intestinal brush border membrane vesicles. A model of high affinity transport (K_m approximating 0.1 mmol/L) converged to the data obtained for ³⁵S-L-Met uptake under Na⁺-gradient and Na⁺-free conditions. The maximal velocity of ³⁵S-L-Met transport was almost threefold greater in the presence of a Na⁺-gradient. Concentrations (100 mmol/L) of D-Met, the L-isomers of neutral amino acids and 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid completely inhibited ³⁵S-L-Met transport. A model of low affinity competitive inhibition (K_i 17 mmol/L) described D-Met inhibition of ³⁵S-L-Met transport. These data indicate that L- and D-Met are transported by a broad specificity system B type transporter. Maximal rates of ³H-L-HMB uptake were obtained under conditions of an internally directed H⁺-gradient (pH_in = 7.5, pH_out = 5.5). A model of intermediate affinity transport (K_m 1 mmol/L) described H⁺-dependent ³H-L-HMB uptake across the vesicles. The data from this and other studies indicate that a H⁺-dependent, nonstereospecific system facilitates the uptake of the hydroxy analogues of linear amino acids, including HMB.

KEY WORDS: • methionine • methionine hydroxy analog • intestinal transport • brush border membrane • chicks

¹ Presented in part at the 83rd Annual Meeting of the Poultry Science Association August 7–12, 1994, Starkville, MS [Maenz, D. D., Engele, C. M. & Classen, H. L. (1994) Transport of methionine (Met) and hydroxymethylthiobutanoic acid (HMB) in the chick intestinal brush border membrane. Poult. Sci. 73: 19 (abs.)].

² Supported by the Degussa Corporation, Ridgefield Park, NJ 07660.

³ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

⁴ To whom reprint requests should be addressed.

Manuscript received 17 April 1995. Revision accepted 27 October 1995.

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