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Antihypertensive Peptides Are Present in Aorta after Oral Administration of Sour Milk Containing These Peptides to Spontaneously Hypertensive Rats^1,2,

R&D Center, The Calpis Food Industry Co., Ltd., Fuchinobe, Sagamihara, Kanagawa 229, Japan

Absorption of inhibitory peptides to angiotensin I-converting enzyme, L-valyl-L-prolyl-L-proline and L-isoleucyl-L-prolyl-L-proline, was studied in spontaneously hypertensive rats and normotensive Wistar-Kyoto rats after an oral administration of Calpis^TM sour milk, which contains these peptides. Six hours after the administration of Calpis^TM sour milk or saline to spontaneously hypertensive rats or normotensive Wistar-Kyoto rats, the blood pressure was measured and rats were killed. Abdominal aorta, lung, kidney, heart and brain were excised, homogenized and solubilized by detergent treatments. Angiotensin I-converting enzyme activity in the solubilized fractions from the abdominal aorta was significantly lower in spontaneously hypertensive rats given the sour milk than in the rats given saline. L-Valyl-L-prolyl-L-proline and L-isoleucyl-L-prolyl-L-proline were detected by HPLC in the heat-treated solubilized fraction from the abdominal aorta of spontaneously hypertensive rats given the sour milk. In contrast, the tripeptides were not detected in rats given saline, or in normotensive Wistar-Kyoto rats given the sour milk or saline. These data suggest that L-valyl-L-prolyl-L-proline and L-isoleucyl-L-prolyl-L-proline in the Calpis^TM sour milk are absorbed directly without being decomposed by digestive enzymes, reach the abdominal aorta, inhibit the angiotensin I—converting enzyme, and show antihy-pertensive effects in spontaneously hypertensive rats.

KEY WORDS: • antihypertensive peptide • fermented milk • angiotensin I-converting enzyme • spontaneously hypertensive rats • intestinal absorption

¹ Presented in abstract form at the plenary meeting of the Japanese Society of Nutrition and Food Science, 1995, Gifu, Japan [Masuda, O., Nakamura, Y. & Takano, T. (1995) Detection of ACE inhibitory peptides from organs after oral administration of sour milk containing these peptides (in Japanese)].

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ To whom correspondence and reprint requests should be addressed.

Manuscript received 4 March 1996. Revision accepted 20 August 1996.

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