QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Joseph, T. Articles by McCormick, D. B. Search for Related Content PubMed PubMed Citation Articles by Joseph, T. Articles by McCormick, D. B.

Pyridoxine 4'-- and 5'--D-Glucosides are Taken Up and Metabolized by Isolated Rat Liver Cells^1,2,

Theresa Joseph^*, Haruhito Tsuge^,3, Yukio Suzuki^** and Donald B. McCormick^*,4

^* Department of Biochemistry, Emory University, Atlanta, GA 30322-3050 Institute for Food Science, Gifu University, Gifu 501-11, Japan ^** Research Institute for Bioresources, Okayama University, Kurashiki 710, Japan

Uptake and metabolism of pyridoxine-4'--D-glucoside and pyridoxine-5'--D-glucoside by freshly isolated rat liver cells were studied using the membrane filtration method. The presence of the pyridoxine--glucosides at either 0.5 or 5.0 µmol/L had no inhibitory effect on the uptake of pyridoxine (0.5 µmol/L) by hepatocytes. The amount of pyridoxine--glucoside transported into the cells was only 10–12% of that of pyridoxine. The initial rates of uptake of the pyridoxine--glucosides were linear with concentrations up to 50 µmol/L. On entry into the cells, 78% of pyridoxine-5'--glucoside and 15% of the pyridoxine-4'--glucoside were hydrolyzed at the end of 60 min. Because pyridoxine-5'--glucoside is hydrolyzed readily and does not interfere with the transport of pyridoxine, the glucoside, if present in animal- or plant-derived foods, may be available as a source of vitamin B-6.

KEY WORDS: • pyridoxine glucoside • pyridoxine • rat hepatocytes • vitamin B-6 • vitamin B-6 metabolism

¹ Supported by the National Institutes of Health (DK 43005), the Huguley Memorial Endowment, and the Coca-Cola Foundation.

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ Short-term visiting professor for specific research (Life Science and Biotechnology) sponsored by Japanese Ministry of Education.

⁴ To whom correspondence should be addressed.

Manuscript received 28 May 1996. Revision accepted 2 August 1996.