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Selection for Body Composition Does Not Affect Energetic Efficiency of Jejunal Glucose Uptake in Mice^1,2,

Yang-Kwang Fan³, Warren J. Croom, Jr.^4,5,, Linda R. Daniel, Anthony R. Bird⁵, Betty L. Black^*, Eugene J. Eisen and Brian W. McBride

Department of Animal Science ^* Department of Zoology, North Carolina State University, Raleigh, NC 27695 Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, N1G2W1 Canada

Five-wk-old male mice from three lines were used to examine whether the apparent energetic efficiency of active jejunal glucose uptake in mouse jejunum is altered by genetic selection for different body composition. The mice lines were selected as follows: HE, high percentage of body fat with no change in body weight as a constraint; LF, low percentage of body fat; and RS, randomly bred control. Body weight was similar in all lines. Total jejunal O₂ consumption and ouabain-sensitive O₂ consumption were used to estimate the energy expenditure associated with glucose absorption and Na⁺/K⁺-ATPase activity. Tritiated 3-O-methyl-D-glucose was used to determine glucose uptake by mouse jejunum. Line LF, when compared with line HE, had lower body fat as indicated by epididymal fat pad weight (143 vs. 362 mg/mouse, P < 0.001). There were no significant differences in small intestinal weight, length and density (mg/cm) between LF and HE lines. Jejunal villus width was greater in line LF compared with line HE (115 vs. 92 µm, P < 0.002). Jejunal glucose transport and O₂ consumption were not different between LF and HE lines. Ouabain-sensitive O₂ consumption was not significantly different among the three lines. No differences were noted in the apparent energetic efficiency of active glucose uptake among lines.

KEY WORDS: • genetic selection • body composition • intestinal absorption • energetic efficiency • mice

¹ The use of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service of the products named or similar ones not mentioned.

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ Present address: Department of Animal Science, National Chung-Hsing University, Taichung, Taiwan.

⁴ Present address: Department of Poultry Science.

⁵ Present address: CSIRO, Division of Human Nutrition, Glenthorne Laboratory, Majors Road, O'Halloran Hill, S. Australia 5158.

⁶ To whom correspondence and reprint requests should be addressed.

Manuscript received 20 November 1995. Revision accepted 7 July 1996.