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Temporal-Regulation of Serum Lipids and Stearoyl CoA Desaturase and Lipoprotein Lipase mRNA in BALB/cHnn Mice^1,2,

Department of Internal Medicine, Division of Nutritional Sciences, University of Illinois College of Medicine, Urbana, IL 61801 ^* Molecular Genetics in Nutrition Program, The Sapient's Institute, Dallas, Texas 75209

Databases for genes expressed in humans or cell cultures are being developed as a part of the Human Genome Project. Because genomes respond to nutritional and other environmental variables, quantitative analyses of mRNA abundance under defined nutritional and physiological states are required to understand normal metabolism and to clarify differences between normal and disease phenotypes. Reported here are comparisons of food intake, growth, serum lipids and expression of mRNA for hepatic stearoyl CoA desaturase (Scd1) and heart lipoprotein lipase (Lpl) in female BALB/cHnn mice following food deprivation and refeeding at the end of 2 wk of feeding semipurified diets with 3, 10 or 20% corn oils. Body weights and utilization of dietary energy were similar for mice fed all three diets. There were no differences in serum lipid concentrations associated with the level of dietary fat during subsequent food deprivation and refeeding, but significant differences in serum triglycerides and total serum cholesterol were observed between food-deprived and fed mice. Heart lipoprotein lipase and hepatic Scd1 mRNA expression levels were affected significantly by concentration of corn oil and by time after eating. These and other studies examining gene regulation by dietary variables and nutrient availability are discussed in relation to development of diet-regulated gene databases for laboratory animals fed semipurified diets.

KEY WORDS: • BALB/cHnn mice • serum lipids • lipoprotein lipase • stearoyl CoA desaturase • diet-regulated gene database

¹ Supported by U.S. Department of Agriculture grant 91-01631, grant NCB93B63 from the American Institute for Cancer Research, a gift from Abbott Laboratories (Columbus, OH), in-kind donations from M&M/MARS, Inc. (Hackettstown, NJ), and the University of Illinois Colleges of Medicine and Agriculture. DAS and EAP were supported by predoctoral fellowships from the American Institute of Nutrition. DAS was also supported by National Institutes of Health traineeship PHS ST32-AM07497.

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ Contributions of these authors were similar.

⁴ Current address: Division of Gastroenterology, Washington University School of Medicine, St. Louis, MO 63110.

⁵ Current address: Department of Food Science and Human Nutrition, Division of Nutritional Sciences, University of Illinois, Urbana, IL.

⁶ To whom correspondence should be addressed at: The Sapient's Institute, c/o University of Illinois College of Medicine, 506 S. Matthews, Urbana, IL 61801.

Manuscript received 29 March 1996. Revision accepted 12 July 1996.