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Methionine-Containing Peptides Can Be Used As Methionine Sources for Protein Accretion in Cultured C₂C₁₂ and MAC-T Cells^1,2,

Yuanlong Pan^*, Patrick K. Bender, R. Michael Akers^** and Kenneth E. Webb, Jr.^*,4

^* Departments of Animal and Poultry Sciences Biochemistry and Anaerobic Microbiology ^** Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0306

Twenty-two methionine-containing di- to octapeptides were evaluated for their ability to be a source of methionine to support protein accretion in C₂C₁₂ myogenic and MAC-T bovine mammary epithelial cells. The cell cultures were incubated for 72 h at 37°C in a humidified environment of 90% air:10% CO₂ for C₂C₁₂ cells or 95% air: 5% CO₂ for MAC-T cells. The basal medium contained methionine-free Dulbecco's modified Eagle's medium and 6% desalted fetal bovine serum. Treatments included basal medium, the basal medium supplemented with one of the 22 methionine-containing peptides, or the basal medium supplemented with free L-methionine. Methionine-containing peptides with the exception of glycylmethionine and prolyimethionine in C₂C₁₂ cells were able to support protein accretion with responses ranging from 29.1 to 123.3% of the response of L-methionine. Dipeptides with methionine at the N-terminus promoted greater (P < 0.0001) protein accretion than dipeptides with methionine at the C-terminus. Stimulation of protein accretion by seven pairs of dipeptides with methionine at either the C- or the N-terminus was linearly (P < 0.0001) related to the hydrophobicity of the dipeptides. These results indicate that C₂C₁₂ myogenic and MAC-T mammary epithelial cells have the ability to utilize methionine-containing peptides as sources of methionine to support protein accretion.

KEY WORDS: • methionine • peptide • protein • myogenic • mammary

¹ Presented in part at Experimental Biology 93, March 28–April 1, 1993, New Orleans, LA [Pan, Y. L., Bender, P. K., Akers, R. M., Turner, J. D. & Webb, K. E., Jr. (1993) Methionine-containing peptides as methionine sources for protein accretion in cultured myogenic and mammary epithelial cells. FASEB J. 7:A523 (abs.)].

² Supported in part by the John Lee Pratt Animal Nutrition Program, Virginia Polytechnic Institute and State University. Support was also provided by the Virginia Agriculture Experiment Station and the Cooperative State Research Service, U.S. Department of Agriculture, under Project No. 6129990.

³ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

⁴ To whom correspondence should be addressed.

Manuscript received 30 March 1995. Revision accepted 7 September 1995.