QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ushijima, K. Articles by Kretchmer, N. Search for Related Content PubMed PubMed Citation Articles by Ushijima, K. Articles by Kretchmer, N.

Absorption of Fructose by Isolated Small Intestine of Rats is Via a Specific Saturable Carrier in the Absence of Glucose and by the Disaccharidase-Related Transport System in the Presence of Glucose^1,2,

Department of Nutritional Sciences, University of California, Berkeley, CA 94720

Previous studies have shown that the absorption of fructose is aided by simultaneous ingestion of glucose. The aim of the present study was to reproduce this finding in vitro to better understand the mechanism of the effect of glucose on absorption of fructose. The phenomena could not be reproduced with everted sleeves of rat intestine or brush border vesicles. In a perfused segment of isolated intestine, it was possible to demonstrate that the transport of fructose was accelerated when glucose was present in the perfusion medium. The enhanced transport was inhibited by sucrose and also by acarbazone, an inhibitor of intestinal -disaccharidases. Phlorizin had no effect on the transport of fructose. The results of these studies indicate that there is a specific carrier for fructose saturated with a low concentration of the sugar, and that in the presence of glucose there is joint absorption of the two sugars by the disaccharidase-related transport system.

KEY WORDS: • fructose • absorption • rats • isolated intestine

¹ Supported in part by NIH grant 5T32HD07266. K. Ushijima received support from a fellowship donated by Osmotica Foods, Davis, California. T. Fujisawa received support from a fellowship donated by A.E. Staley Manufacturing, Decatur, Illinois.

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ To whom correspondence should be addressed.

Manuscript received 22 August 1994. Revision accepted 9 February 1995.