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Copper Deficiency Alters Rat Peptidylglycine -Amidating Monooxygenase Activity^1,2,

Department of Biochemistry and Molecular Biology, University of Minnesota, Duluth, MN 55812

Perinatal copper deficiency was studied in 1-mo-old female and male Sprague-Dawley rat offspring to investigate changes in cuproenzymes. Offspring of dams given the low Cu treatment beginning at d 7 of gestation exhibited signs characteristic of Cu deficiency, including a 90% reduction in liver Cu levels compared with Cu-adequate controls. Compared with Cu-adequate rats, Cu-deficient rats had lower activities of the cuproenzymes peptidylglycine -amidating monooxygenase (PAM), cytochrome c oxidase (CCO), and Cu,Zn-superoxide dismutase (SOD) in heart and midbrain samples. Activity of dopamine-ß-monooxygenase (DBM) was higher in midbrain and lower in heart samples from Cu-deficient compared with Cu-adequate rats. Following 1 mo of Cu repletion, PAM and CCO activity were still lower in heart of Cu-replete rats. Midbrain DBM activity was still elevated in the former Cu-deficient males. A second study was conducted using weanling male Holtzman rats. After 5.5 wk of treatment, Cu-deficient rats had signs characteristic of Cu deficiency and lower PAM, CCO and DBM activities in heart but not midbrain as compared with Cu-adequate rats. The PAM activity was lower following Cu deficiency. Perhaps neuropeptide maturation is compromised by Cu deficiency.

KEY WORDS: • copper deficiency • cuproenzymes • peptidylglycine -amidating monooxygenase • rats

¹ Supported by grant 93-37200-8756 from NRI Competitive Grants Program/USDA.

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ To whom correspondence should be addressed.

Manuscript received 3 October 1994. Revision accepted 5 January 1995.

This article has been cited by other articles:

				W. T. Johnson and C. M. Anderson Cardiac Cytochrome c Oxidase Activity and Contents of Subunits 1 and 4 Are Altered in Offspring by Low Prenatal Copper Intake by Rat Dams J. Nutr., July 1, 2008; 138(7): 1269 - 1273. [Abstract] [Full Text] [PDF]