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¹⁵N-Labeled Immunoglobulins from Bovine Colostrum Are Partially Resistant to Digestion in Human Intestine^1,2,3,

N. Roos⁴, S. Mahé^*, R. Benamouzig, H. Sick, J. Rautureau and D. Tomé^*

Institut für Physiologie und Biochemie der Ernährung, Bundesanstalt für Milchforschung, D-24121 Kiel, Germany ^* Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 75006 Paris, France Service de Gastroentérologie, Hôpital Avicenne, 93009 Bobigny Cedex, France

To evaluate true ileal digestibility of bovine immunoglobulins, seven healthy human adults ingested a ¹⁵N-labeled preparation of an immunoglobulin concentrate. After fasting overnight, subjects drank 400 mL of immunoglobulin concentrate (77 mmol), and ileal effluents were collected for 8 h at 20-min intervals using a naso-intestinal intubation technique. In addition to osmolality and pH, and concentrations of exogenous and endogenous nitrogen and ions (Na⁺, K⁺, Cl^-, Ca²⁺, Mg²⁺) in the effluents were measured. Bovine immunoglobulin concentrations (IgG, IgM, IgA) were estimated by a radial immunodiffusion technique. The mean flow rate of the liquid phase was 22.3 ± 6.1 mL/20 min and did not vary significantly during the collection period. No change was observed for osmolality, pH or Na⁺, K⁺ and Cl^- concentrations. Two hours after meal ingestion, Ca²⁺ and Mg²⁺ concentrations increased significantly (P < 0.05). The recoveries of nitrogen of ingested IgG and IgM still immunologically active were 19 ± 3% and 19 ± 4%, respectively. No IgA was detected in the ileum. Mean digestibility of the exogenous nitrogen fraction was 79 ± 3%. In comparison to literature data, which show that other milk proteins have ileal digestibilities of >90%, our results demonstrate a lower ileal digestibility of bovine immunoglobulins in humans.

KEY WORDS: • immunoglobulins • digestibility • humans • stable isotopes

¹ Presented in part at the XV International Congress of Nutrition, Adelaide, Australia, September 26–October 1, 1993 [Roos, N., Mahé, S., Benamouzig, R., Sick, H., Rautureau, J. & Tomé, D. (1993) Ileal absorption of ¹⁵N-labelled bovine colostrum immunoglobulins in humans. Abstracts, Book 1, p. 107, 447].

² The stay of N. Roos in France during this study was supported by a grant of the EU program PROCOPE (grant no. 311/312-pro).

³ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

⁴ To whom correspondence should be addressed.

Manuscript received 10 June 1994. Revision accepted 18 November 1994.