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Pyridoxine-5'-ß-D-Glucoside Influences the Short-Term Metabolic Utilization of Pyridoxine in Rats^1,2,3,

Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611-0370

This study was conducted to characterize the initial time course of the apparent competitive effect of pyridoxine-5'-ß-D-glucoside against co-ingested pyridoxine. Two groups of rats were administered a single oral dose of 100 nmol of [¹⁴C]pyridoxine along with either 0 or 20 nmol of unlabeled pyridoxine-5'-ß-D-glucoside. At 6, 12, 24 and 48 h post-dose, the distribution of labeled vitamin B-6 metabolites in blood, tissues and urine was determined. Urinary [¹⁴C]4-pyridoxic acid comprised a significantly greater percentage of excreted ¹⁴C in the control group, with the greatest difference at 12 h post-dose. Pyridoxine-5'-ß-D-glucoside (10–15 nmol) was excreted mainly in unchanged form within 6 h. Rats that received pyridoxine-5'-ß-D-glucoside retained less ¹⁴C in liver, with a maximal difference between groups at 6–12 h post-dose. The relative concentrations of hepatic [¹⁴C]pyridoxal 5'-phosphate and [¹⁴C]pyridoxamine 5'-phosphate in the treatment group were greater than in the control group at 12 h post-dose. At 48 h post-dose, there was no difference in the distribution of any vitamin B-6 metabolite except pyridoxal 5'-phosphate in the two groups. These results confirm that a small, nutritionally relevant dose of pyridoxine-5'-ß-D-glucoside influences the utilization of pyridoxine and indicate that this is a short-term, transient effect.

KEY WORDS: • vitamin B-6 • pyridoxine glucoside • metabolic utilization • rats

¹ Presented in abstract form at Experimental Biology 94, April 1994, Anaheim, CA [Nakano, H. & Gregory, J. F. (1994) The influence of pyridoxine glucoside on vitamin B6 metabolism in rats. FASEB J. 8: A704 (abs.)].

² Supported by grant no. DK37481 from the National Institutes of Health. Florida Agricultural Experiment Station journal series no. R-03862.

³ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

⁴ To whom correspondence should be addressed.

Manuscript received 10 June 1994. Revision accepted 30 August 1994.