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Prediction of Liver Vitamin A in Rats by an Oral Isotope Dilution Technique^{1, 2,}

Nutrition Department, The Pennsylvania State University, University Park, PA 16802

Male Sprague-Dawley rats (n = 41) that had liver vitamin A levels ranging from 5 nmol to 22 µmol (0.48 to 1629 nmol/g) were intubated with [³H]retinol in oil (167 kBq/rat). Plasma samples collected at 3, 4, 4.4, 5, 5.4 and 6 d were analyzed for radioactivity, and liver vitamin A mass was determined at 8 d. Nonlinear regression analysis was used to develop a biexponential equation describing the relationship at each sampling time between fraction of the dose remaining in plasma (FD_p) and liver vitamin A. The best equation, developed with 3-d data, was liver vitamin A (nmol) = 58,577 x exp(-2715 x FD_p) + 1810 x exp(-127 x FD_p); Spearman's rank correlation coefficient = 0.982. This equation showed good predictive ability as evidenced by the ratio of the calculated to the observed liver vitamin A (1.23 ± 0.65) and by its ability to distinguish animals with deficient, marginal, adequate or high vitamin A status. We conclude that this sensitive, noninvasive procedure can be used to predict rat liver vitamin A over a wide range.

KEY WORDS: • vitamin A assessment • liver vitamin A • retinol • isotope dilution • rats

¹ Supported by U.S. Department of Agriculture Competitive Research Grant 88-37200-3537.

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ To whom correspondence should be addressed.

Manuscript received 29 September 1993. Revision accepted 17 February 1994.

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