QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Phillips, F. C. Articles by Cleary, M. P. Search for Related Content PubMed PubMed Citation Articles by Phillips, F. C. Articles by Cleary, M. P.

Metabolic Measurements among Homozygous (fa/fa) Obese, Heterozygous (Fa/fa) Lean and Homozygous (Fa/Fa) Lean Zucker Rat Pups at 17 Days of Age^{1, 2,}

The Hormel Institute, University of Minnesota, Austin, MN 55912

Factors associated with the development of obesity were compared among obese (fa/fa), heterozygous (Fa/fa) lean and homozygous (Fa/Fa) lean Zucker rats at 17 d of age. Inguinal pad weight, pad-to-body weight ratio and fat cell size were highest in obese pups (fa/fa > Fa/fa > Fa/Fa). Hepatic glucose-6-phosphate dehydrogenase activity was greater in fa/fa than in Fa/Fa pups; 6-phosphogluconate dehydrogenase activity was higher in fa/fa and Fa/fa pups than in Fa/Fa pups, and fatty acid synthetase was greater in fa/fa compared with lean pups (Fa/fa = Fa/Fa). The fa/fa pups had greater adipose tissue glucose-6-phosphate dehydrogenase, malic enzyme and fatty acid synthetase activities than lean pups, which did not differ from one another (Fa/fa = Fa/Fa), whereas 6-phosphogluconate dehydrogenase and lipoprotein lipase activities were highest in obese pups, intermediate in heterozygotes and lowest in homozygous lean rats (fa/fa > Fa/fa > Fa/Fa). Glucose conversion to carbon dioxide and fatty acids in isolated adipocytes was highest in obese pups (fa/fa > Fa/fa > Fa/Fa). Glyceride-glycerol production was greater in Fa/Fa than in fa/fa or Fa/fa pups. These findings indicate that many characteristics of obesity are evident in preobese Zucker rats, and for some factors the presence of the "fa" gene in lean rats results in intermediate measurements relative to the two homozygous genotypes.

KEY WORDS: • glucose metabolism • lipogenic enzymes • liver • adipose tissue • suckling rats

¹ Supported by National Institutes of Health grant HD25006 and by The Hormel Foundation.

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ To whom correspondence should be addressed.

Manuscript received 23 November 1993. Revision accepted 21 February 1994.

This article has been cited by other articles:

				S. K. Iyengar, C. M. Stein, K. Russo, B. O. Erokwu, and K. P. Strohl The fa leptin receptor mutation and the heritability of respiratory frequency in a Brown Norway and Zucker intercross J Appl Physiol, September 1, 2004; 97(3): 811 - 820. [Abstract] [Full Text] [PDF]