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Lysine--Ketoglutarate Reductase and Saccharopine Dehydrogenase Are Located Only in the Mitochondrial Matrix in Rat Liver¹

Ken P. Blemings^*,, Thomas D. Crenshaw^*, Robert W. Swick and Norlin J. Benevenga^*,,2

^* Department of Meat and Animal Science Department of Nutritional Sciences, University of Wisconsin, Madison, WI 53706

In rat liver, comparisons of marker enzyme activities (ß-hexoseaminidase, lysosomes; catalase, peroxisomes; cytochrome oxidase, mitochondrial-inner membrane; monoamine oxidase, mitochondrial outer membrane; ornithine aminotransferase, mitochondrial matrix) show that lysine--ketoglutarate reductase and saccharopine dehydrogenase, the initial enzymes of saccharopine-dependent lysine degradation, are found only in the mitochondrial matrix. These results are consistent with obligatory uptake of lysine into the matrix for lysine catabolism and raise the possibility that lysine transport into the mitochondrion may control lysine degradation.

KEY WORDS: • lysine • mitochondria • rats • lysine--ketoglutarate reductase • saccharopine dehydrogenase

¹ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

² To whom correspondence should be addressed.

Manuscript received 29 October 1993. Revision accepted 16 February 1994.

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