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Determination of Fractional Absorption of Dietary Calcium in Humans¹

Alfred L. Yergey², Steven A. Abrams^*, Nancy E. Vieira, Akram Aldroubi, Joan Marini and James B. Sidbury

National Institute of Child Health and Human Development, Bethesda, MD 20892, ^* USDA/ARS Children's Nutrition Research, Center, Houston, TX, Biomedical Engineering Program, National Institutes of Health, Bethesda, MD 20892

Four dual-isotopic label methods for determining true fractional absorption of dietary calcium were compared in 23 subjects. The ratio of the integrals of oral label in a 24-h pooled urine to intravenous label in the same urine is called _24h and was taken as the standard against which the others were compared. _Spot is the ratio of the fraction of oral label to the fraction of intravenous label in a single urine specimen; _Lag is the ratio of the level of oral label in blood 4 h after administration to the level of intravenous label in blood 2 h after administration. _Dec is obtained by deconvoluting response to the intravenous label from the response to the oral tracer. Results were as follows: _24h = 0.273 ± 0.124, _Dec = 0.300 ± 0.101 (n = 14), _Spot = 0.359 ± 0.179, and _Lag = 0.271 ± 0.103. The Bland-Altman approach for comparison of methods was used to show that results for _Spot and _Lag can be expected, with a 95% confidence limit, to differ from the value of _24h by 60 and 69%, respectively. The results for _Dec were shown to be not only indistinguishable from _24h but identical from a theoretical perspective.

KEY WORDS: • mass spectrometry • humans • stable isotopes • dietary calcium absorption

¹ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

² To whom correspondence should be addressed.

Manuscript received 27 May 1993. Revision accepted 7 December 1993.

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