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Jejunal Glucose Absorption Is Enhanced by Epidermal Growth Factor in Mice^1,2,

Anthony R. Bird⁴, Warren J. Croom, Jr.⁵, Yang K. Fan, Linda R. Daniel, Betty L. Black^*, Brian W. McBride, Eugene J. Eisen, Leonard S. Bull and Ian L. Taylor^**,6

Department of Animal Science ^* Zoology, North Carolina State University, Raleigh, NC 27695-7621 Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, N1G 2W1, Canada The^** Durham VAMC and the Department of Medicine, Division of Gastroenterology, Duke University Medical Center, Durham, NC 27710

The effects of epidermal growth factor on intestinal glucose transport were examined in mice. Glucose transport measurements were performed using an in vitro assay system that estimated the rate of accumulation of [³H]3-O-methyl-D-glucose. In Experiment 1, two-mo-old male and female mice were subcutaneously injected once daily with 0, 150 or 300 µg epidermal growth factor/kg body weight for 3 d. Jejunal glucose active transport was increased in a dose-dependent manner. There were no gender-related differences in intestinal glucose transport or the response to exogenous epidermal growth factor. In Experiment 2, 2-, 10- and 18-mo-old mice were administered 0 or 300 µg epidermal growth factor/kg body weight using a treatment similar to that used in Experiment 1. Active intestinal glucose transport was 30% greater in response to epidermal growth factor in each of the three age groups. Ouabain-sensitive and -insensitive jejunal oxygen consumption was increased in response to epidermal growth factor such that total jejunal respiration was stimulated 15 to 31%. The epidermal growth factor related percentage increase in glucose absorption was similar to the percentage increase in oxygen consumption such that the apparent energetic efficiency of glucose transport was unaffected. In both experiments, the active component of glucose transport was increased by epidermal growth factor while passive transport was not affected. Jejunal morphology and mucosal DNA and protein concentration were not altered by epidermal growth factor treatment. Epidermal growth factor-induced increases in intestinal absorption was not attributable to mucosal hyperplasia.

KEY WORDS: • epidermal growth factor • glucose • absorption • intestine • mice

¹ Presented in part at the 1992 Annual Meeting of the American Gastroenterological Association, May 10–13, 1992, San Francisco, CA [Bird, A. R., Croom, W. J., Jr., Daniel, L. R., Black, B. L. & Eisen, E. J. (1992) Intestinal glucose transport in mice treated with EGF. Gastroenterology 102: A543 (abs.)].

² The use of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service of the products named or similar ones not mentioned.

³ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

⁴ Recipient of an Australian Wool Research and Development Corporation Postgraduate Research Scholarship and supported in part by the Queensland Department of Primary Industries.

⁵ To whom correspondence and reprint requests should be addressed.

⁶ Supported by NIH grants DK 38216 and DK 44072. Current address: Department of Medicine, Medical University of South Carolina, Charleston, SC 29425-2220.

Manuscript received 21 May 1993. Revision accepted 16 September 1993.

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