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Dietary Fat Saturation and Endurance Exercise Alter Lipolytic Sensitivity of Adipocytes Isolated from Yucatan Miniature Swine^1,2,3,

Department of Animal and Nutritional Sciences, University of New Hampshire, Durham, NH 03824

This study examined the effects of dietary fat saturation and endurance exercise on lipolytic sensitivity of adipocytes isolated from Yucatan miniature swine. Twenty-four female swine had free access to a high fat diet with a polyunsaturated to saturated fat ratio (P:S) of 0.3 or 1.0, and were treadmill-exercised or remained sedentary. After 3 months, biopsies were taken, adipocytes were isolated and lipolytic activity was determined. Adipocytes were incubated with adenosine deaminase followed by epinephrine, isoproterenol, or epinephrine plus phenylisopropyladenosine, and glycerol release was measured. Backfat thickness was measured by ultrasonography. Our findings revealed that 1) adipocytes from 1.0 P:S diet-fed swine released 30% more glycerol than adipocytes from 0.3 P:S diet-fed swine when stimulated by 1 µmol/L isoproterenol; 2) adipocytes from exercised swine released 45% more glycerol than adipocytes from sedentary swine when stimulated by 1 µmol/L epinephrine; 3) body weight of exercised swine was significantly lower than sedentary swine; and 4) backfat thickness was less in exercised swine than in sedentary swine (2.39 vs. 2.95 cm, P = 0.002). We conclude that ad libitum consumption of diet with a P:S of 1.0, combined with endurance exercise, increases lipolytic sensitivity, lowers body weight gain, and reduces fat accumulation in female Yucatan miniature swine.

KEY WORDS: • polyunsaturated:saturated • Yucatan swine • lipolysis • exercise • adipocyte

¹ Presented in poster form at the annual meeting of the American College of Sports Medicine, June, 1993, Seattle, WA [Meservey, C. & Carey, G. (1993) Effect of Exercise and two polyunsaturated:saturated fat diets on adipocyte sensitivity to adenosine in swine. Med. Science Sports Exercise 25(5), 530 (abs.)].

² Scientific Contribution number 1850 from the New Hampshire Agricultural Experiment Station.

³ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

⁴ Current address: Dept. of Clinical Sciences, University of Massachusetts-Lowell, Lowell, MA 01854.

⁵ To whom correspondence should be addressed.

Manuscript received 15 December 1993. Revision accepted 1 June 1994.