QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lauterio, T. J. Articles by Ulman, E. A. Search for Related Content PubMed PubMed Citation Articles by Lauterio, T. J. Articles by Ulman, E. A.

Development and Characterization of a Purified Diet to Identify Obesity-Susceptible and Resistant Rat Populations^1,2,

Departments of Internal Medicine and Physiology, Eastern Virginia Medical School, Norfolk, VA 23501 Department of Veterans Affairs Medical Center, Hampton, VA 23667 ^* Research Diets Incorporated, New Brunswick, NJ 08901

A purified moderately high fat diet has been developed to examine diet-induced obesity in rats. Male Sprague-Dawley rats were fed this or an AIN-76A diet for 15 wk and energy metabolism indices were monitored. Food intake, body weight and water balance indices were recorded on a weekly or daily basis. Over the 15-wk period, rats fed the experimental diet diverged into two groups differing in the rate of body weight gain. Animals were labeled as "gainers" or "resisters" depending on their susceptibility to obesity. Following the dietary period, rats were decapitated and trunk blood was collected for glucose and insulin measurements. Gainers consumed slightly more energy than resisters over the experimental period (P < 0.05), but due to greater fecal energy loss, absorbed energy did not differ. Hence gainers became obese without significantly altered energy retention. Urinary creatinine, urea nitrogen and water balance were not different between the groups and consequently could not explain body weight differences. Further, gainers had significantly greater plasma glucose concentration than controls, indicating a potential for these animals to become diabetic. Results suggest metabolic differences must account for the divergence in weight gain observed in the two groups. The dietary model characterized in this study should provide a useful tool to study diet-induced obesity and to determine its underlying mechanism.

KEY WORDS: • diet-induced obesity • feed efficiency • rats • resisters and gainers • glucose concentrations

¹ This work was supported by NIH grant DK40982 to TJL.

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ To whom correspondence should be addressed.

Manuscript received 24 January 1994. Revision accepted 5 May 1994.

This article has been cited by other articles:

				F. Zammaretti, G. Panzica, and C. Eva Sex-dependent regulation of hypothalamic neuropeptide Y-Y1 receptor gene expression in moderate/high fat, high-energy diet-fed mice J. Physiol., September 1, 2007; 583(2): 445 - 454. [Abstract] [Full Text] [PDF]

				J. N. Gorski, A. A. Dunn-Meynell, and B. E. Levin Maternal obesity increases hypothalamic leptin receptor expression and sensitivity in juvenile obesity-prone rats Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R1782 - R1791. [Abstract] [Full Text] [PDF]

				J. N. Gorski, A. A. Dunn-Meynell, T. G. Hartman, and B. E. Levin Postnatal environment overrides genetic and prenatal factors influencing offspring obesity and insulin resistance Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2006; 291(3): R768 - R778. [Abstract] [Full Text] [PDF]

				J. F. Carroll, W. J. Zenebe, and T. B. Strange Cardiovascular Function in a Rat Model of Diet-Induced Obesity Hypertension, July 1, 2006; 48(1): 65 - 72. [Abstract] [Full Text] [PDF]

				K. A. Singh, C. N. Boozer, and J. R. Vasselli Acute insulin-induced elevations of circulating leptin and feeding inhibition in lean but not obese rats Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2005; 289(2): R373 - R379. [Abstract] [Full Text] [PDF]

				A. Gomori, A. Ishihara, M. Ito, S. Mashiko, H. Matsushita, M. Yumoto, M. Ito, T. Tanaka, S. Tokita, M. Moriya, et al. Chronic intracerebroventricular infusion of MCH causes obesity in mice Am J Physiol Endocrinol Metab, March 1, 2003; 284(3): E583 - E588. [Abstract] [Full Text] [PDF]