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Gastric Emptying Regulates the Kinetics of Nitrogen Absorption from ¹⁵N-Labeled Milk and ¹⁵N-Labeled Yogurt in Miniature Pigs^1,2,

Claire Gaudichon, Nils Roos^*, Sylvain Mahé³, Hinrich Sick^*, Christine Bouley and Daniel Tomé

Institut National de la Recherche Agronomique, Unité de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 75006 Paris, France ^* Institut für Physiologie und Biochemie der Ernährung, Bundesanstalt für Milchforschung, PO-Box 6069, 24121 Kiel, Germany DANONE, 15 avenue Galilée, 92350 Le Plessis Robinson, France

Thirty-six miniature pigs divided into two groups of 18 animals were fed ¹⁵N-labeled milk or yogurt. Polyethylene glycol 4000 was added to the diets as a non-absorbable marker of the liquid phase. Animals were slaughtered 1, 2, 4, 8 and 12 h after meal ingestion, and the gastrointestinal tract was removed and divided into 10 parts. Polyethylene glycol, total nitrogen and ¹⁵N enrichment were measured in the digesta. Both the intestinal delivery of the liquid phase and the nitrogenous fraction of the chyme were delayed more in pigs fed yogurt than in pigs fed milk. No stimulatory effect of diet ingestion on endogenous nitrogen secretion was found. Both milk proteins and yogurt proteins were highly digestible: 93% of the exogenous nitrogen disappeared 12 h after feeding. The kinetics of exogenous nitrogen delivery into the intestine was correlated (r = 0.999 for milk and r = 0.974 for yogurt) with that of exogenous nitrogen absorption. These results suggest that milk proteins are rapidly absorbed after they reach the intestine. Gastric emptying is a major factor controlling the kinetics of milk nitrogen absorption.

KEY WORDS: • pigs • ¹⁵N • milk • yogurt • digestion

¹ Supported in part by grant 90 G 0342 from the French Department of Research and Space. The stay of French scientists in Kiel was supported in part by the EEC program Procope.

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ To whom correspondence should be addressed.

Manuscript received 31 December 1993. Revision accepted 5 April 1994.

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