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High Casein-Lactalbumin Diet Accelerates Blood Coagulation in Rats^1,2,3,

Department of Foods and Nutrition, The University of Georgia, Athens, GA 30602 ^* Department of Pharmacology and Toxicology, College of Pharmacy, The University of Georgia, Athens, GA 30602

This study investigates the influence of a high protein intake on normal hemostasis, fluid balance and organ growth. Adult rats were fed semipurified diets that contained either 18 or 56 g/100 g casein-lactalbumin for 2 wk, and the following functions were measured: food and water intake, weight gain, blood pressure, bleeding and clotting time, ADP-stimulated platelet aggregation, thrombin time, prothrombin time and partial thromboplastin time. Although food intake was depressed by the high protein diet, weight gain was not affected by the regimen. Water consumption, 24-h urine excretion and kidney weight were significantly greater in rats fed the high protein diet than in controls. High protein intake resulted in shorter barbiturate-induced sleeping time. Bleeding time and clotting time were significantly lower in rats fed the high protein diet for 7 d. However, heart rate, mean arterial pressure, plasma protein and osmolarity, platelet aggregation, prothrombin time, partial thromboplastin time and thrombin time did not differ significantly. Because a high protein intake caused rapid coagulation of blood in rats without affecting the activity of clotting factors, we suggest that this diet sensitized rats to factors that initiate clotting in vivo.

KEY WORDS: • dietary proteins • bleeding time • blood coagulation • platelet aggregation • rats

¹ Supported by funds from the Georgia Agriculture Experiment Station, Hatch Act no. H633.

² This work is submitted in partial fulfillment of the doctoral requirements for Kung-Chi Chan.

³ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

⁴ To whom correspondence should be addressed.

Manuscript received 3 November 1992. Revision accepted 8 February 1993.