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[³H]Phytic Acid (Inositol Hexaphosphate) Is Absorbed and Distributed to Various Tissues in Rats^{1, 2,}

Department of Pathology ^* Department of Medical and Research Technology, University of Maryland School of Medicine, Baltimore, MD 21201

To understand the mechanism of antineoplastic action of phytic acid, we investigated the absorption and distribution of myo-[inositol-2-³H(N)]hexakisphosphate in rats. The radioactivity was measured in urine, feces, blood, gastrointestinal tract contents and various organs and tissues at 1 and 24 h after intragastric administration. Of the total radioactivity, 79.0 ± 10.0% was absorbed and at least 26.6% was degraded during the 24-h period following ingestion. The absorption was rapid; 11.0 ± 2.6% of the radioactivity was detected in the wall of the stomach (4.4 ± 3.7%) and upper small intestine (6.6 ± 1.9%), 6.5 ± 2.6% in the skeletal muscle and 4.0 ± 1.5% in the skin after 1 h. Much of the radioactivity after 24 h was in the liver (4.0 ± 0.9%), kidneys (2.2 ± 1.1%), muscle (18.1 ± 3.4%) and skin (10.1 ± 3.3%). Analysis of plasma and urine demonstrated that most of the radioactivity was due to myo-inositol and small amounts of inositol monophosphate (InsP₁). Gastric epithelial cells, however, contained inositol and various inositol phosphates (InsP_1–6). Our data suggest that soluble InsP₆ when administered in drinking water is rapidly absorbed through the stomach and upper small intestine, becomes quickly dephosphorylated within the mucosal cells and is distributed to various organs as inositol and InsP₁.

KEY WORDS: • inositol hexaphosphate • absorption • metabolism • rats • phytic acid

¹ Presented at the Annual Meeting of the Federation of American Societies for Experimental Biology, April 5–9, 1992, Anaheim, CA [Sakamoto, K., Vucenik, I. & Shamsuddin, A. M. (1992) Absorption and tissue distribution of phytic acid (inositol hexaphosphate, InsP₆) in rats. FASEB J. 6: A1392 (abs.)].

² Supported in part by a Designated Research Initiative Fund (DRIF) grant from the University of Maryland. This is contribution no. 3170 from the Pathobiology Laboratory.

³ Current address: Department of Surgery I, Gunma University School of Medicine, Maebashi, Gunma 371, Japan.

⁴ To whom correspondence should be addressed.

Manuscript received 4 August 1992. Revision accepted 30 November 1992.

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