QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sève, B. Articles by Obled, C. Search for Related Content PubMed PubMed Citation Articles by Sève, B. Articles by Obled, C.

Recombinant Porcine Somatotropin and Dietary Protein Enhance Protein Synthesis in Growing Pigs^{1, 2,}

Station de Recherches Porcines, INRA St Gilles, F35590 L'Hermitage ^* Laboratoire d'Etude du Métabolisme Azoté, INRA Theix, F63122 Ceyrat, France

The effects of a daily porcine somatotropin injection on protein synthesis rate in muscle (longissimus), liver and intestine, as influenced by dietary protein, were investigated in 17 pigs. The measurements were made at wk 3 of treatment following 1 wk for adaptation to the diet and 1 wk for determination of nitrogen balance. The fractional rates of protein synthesis in the muscle, liver and intestine were measured using a flooding dose of L-[1-¹³C]valine. Positive responses of weight gain and nitrogen balance were observed, primarily at higher dietary protein intake, after porcine somatotropin treatment. As expected, porcine somatotropin-treated pigs had a higher proportion of muscle and less fat. Fractional protein synthesis rate was 16% higher in the liver of porcine somatotropin-treated pigs (P < 0.05). In the longissimus muscle fractional protein synthesis rate increased with porcine somatotropin dose from 3.2 to 3.7%/d and from 4.1 to 5.1%/d at low and high protein intake, respectively (P < 0.05). The effect of dietary protein on fractional protein synthesis rate in longissimus was significant, but there was no porcine somatotropin x protein interaction. Ribonucleic acid concentration followed the same pattern as fractional protein synthesis rate in liver and longissimus. In the duodenal tissue, porcine somatotropin treatment depressed fractional protein synthesis rate (P < 0.05) without an effect of dietary protein and RNA concentration did not change. In porcine somatotropin compared with placebo-treated pigs, plasma glucose, insulin and insulin-like growth factor-I concentrations were elevated whereas plasma thyroxine was depressed and plasma triiodothyronine remained constant. There was no clear effect of dietary protein on plasma hormones. We concluded that, in pigs fed an adequate level of protein, porcine somatotropin stimulates protein synthesis in the liver and the muscle, primarily through increased ribosomal capacity.

KEY WORDS: • swine • somatotropin • protein synthesis • thyroxin • insulin-like growth factor-I

¹ Presented in part at the 43rd Annual Meeting of the European Association for Animal Production, September 1992, Madrid, Spain, [Sève, B., Ballèvre, O., Obled, C. & Noblet, J. (1992) Protein synthesis in different tissues of 65 kg live weight pigs as influenced by porcine somatotropin and dietary protein. Proc. Eur. Assoc. Anim. Prod. 2: 474 (abs.)].

² Appreciation is expressed to the Monsanto Company (St. Louis, MO) for providing recombinant porcine somatotropin.

³ Current address: Nestec Ltd, Centre de Recherches, Vers-Chez-Les-Blancs, Case postale 44, CH1000 Lausanne 26, Switzerland.

Manuscript received 30 June 1992. Revision accepted 6 November 1992.

This article has been cited by other articles:

				F. A. Wilson, R. A. Orellana, A. Suryawan, H. V. Nguyen, A. S. Jeyapalan, J. Frank, and T. A. Davis Stimulation of muscle protein synthesis by somatotropin in pigs is independent of the somatotropin-induced increase in circulating insulin Am J Physiol Endocrinol Metab, July 1, 2008; 295(1): E187 - E194. [Abstract] [Full Text] [PDF]

				K. Rufibach, N. Stefanoni, V. Rey-Roethlisberger, P. Schneiter, M. G. Doherr, L. Tappy, and J. W. Blum Protein synthesis in jejunum and liver of neonatal calves fed vitamin A and lactoferrin. J Dairy Sci, August 1, 2006; 89(8): 3075 - 3086. [Abstract] [Full Text] [PDF]

				F. Guay, S. M. Donovan, and N. L. Trottier Biochemical and morphological developments are partially impaired in intestinal mucosa from growing pigs fed reduced-protein diets supplemented with crystalline amino acids J Anim Sci, July 1, 2006; 84(7): 1749 - 1760. [Abstract] [Full Text] [PDF]

				J. A. Bush, D. G. Burrin, A. Suryawan, P. M. J. O'Connor, H. V. Nguyen, P. J. Reeds, N. C. Steele, J. B. Van Goudoever, and T. A. Davis Somatotropin-induced protein anabolism in hindquarters and portal-drained viscera of growing pigs Am J Physiol Endocrinol Metab, February 1, 2003; 284(2): E302 - E312. [Abstract] [Full Text] [PDF]