QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Darcy-Vrillon, B. Articles by Duee, P.-H. Search for Related Content PubMed PubMed Citation Articles by Darcy-Vrillon, B. Articles by Duee, P.-H.

Metabolic Characteristics of Pig Colonocytes after Adaptation to a High Fiber Diet^1,2,

Unité d'Ecologie et de Physiologie du Système Digestif ^* Unité de Nutrition et Sécurité Alimentaire, Institut National de la Recherche Agronomique, Centre de Recherches de Jouy-en-Josas, F-78352 Jouy-en-Josas Cedex, France

The capacities of viable colonic epithelial cells to metabolize glucose, glutamine and n-butyrate were studied in 30-kg pigs adapted to a high fiber (12% sugar beet fiber) or a low fiber diet. Glucose and glutamine were extensively utilized but predominantly not oxidized, whereas n-butyrate oxidation accounted for 45% of n-butyrate metabolism and was not greatly affected by the presence of glucose or glutamine. With both diets, glycolysis was the major pathway accounting for glucose disappearance. There was a sparing effect of n-butyrate on both glycolysis and glucose oxidation. Moreover, the glycolytic capacity was 25% lower in pigs fed the high fiber diet. Data suggest that 6-phosphofructo-1-kinase could be the regulatory step in glycolysis. Nevertheless, its maximum activity was not affected by the diet or by the presence of n-butyrate. Glutamine metabolism was slightly affected by fiber in the diet and by the presence of n-butyrate. In addition to CO₂, butyrate was converted into ketone bodies. Glucose and glutamine did not substantially alter n-butyrate metabolism. We conclude that some metabolic features of pig colonocytes, such as the capacity to oxidize n-butyrate, resemble those of rat and human colonocytes. Moreover, some characteristics, such as the glycolytic capacity, can be modulated by the level of fiber in the diet.

KEY WORDS: • colonocytes • metabolism • dietary fiber • pigs

¹ Supported by grant 89-90/4725 from INRA "Actions digestives, fermentaires et métaboliques de l'ingestion de fibres alimentaires."

² Presented in part at the French Association of Nutrition, October 8–9, 1991, Dijon, France and to be published in an abstract form [Darcy-Vrillon, B., Morel, M. T., Cherbuy, C., Bernard, F., Meslin, J. C., Blachier, F. & Duée, P. H. (1992) Fuel metabolism in pig colonocytes after adaptation to a high fibre diet. Reprod. Nutr. Develop. (in press)].

³ To whom correspondence should be addressed.

Manuscript received 22 May 1992. Revision accepted 30 September 1992.

This article has been cited by other articles:

				G. Biagi, A. Piva, M. Moschini, E. Vezzali, and F. X. Roth Performance, intestinal microflora, and wall morphology of weanling pigs fed sodium butyrate J Anim Sci, May 1, 2007; 85(5): 1184 - 1191. [Abstract] [Full Text] [PDF]

				P. Bikker, A. Dirkzwager, J. Fledderus, P. Trevisi, I. le Huerou-Luron, J. P. Lalles, and A. Awati The effect of dietary protein and fermentable carbohydrates levels on growth performance and intestinal characteristics in newly weaned piglets J Anim Sci, December 1, 2006; 84(12): 3337 - 3345. [Abstract] [Full Text] [PDF]

				G. Biagi, A. Piva, M. Moschini, E. Vezzali, and F. X. Roth Effect of gluconic acid on piglet growth performance, intestinal microflora, and intestinal wall morphology J Anim Sci, February 1, 2006; 84(2): 370 - 378. [Abstract] [Full Text] [PDF]