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Soybean Trypsin Inhibitor(s) Reduce Absorption of Exogenous and Increase Loss of Endogenous Protein in Miniature Pigs^1,2,3,

Institut für Physiologie und Biochemie der Ernährung, Bundesanstalt für Milchforschung, D-24103 Kiel, Hermann-Weigmann-Str. 1, Germany

It was the purpose of this study to define whether trypsin inhibitors impair protein digestibility via enhanced loss of exogenous or endogenous protein by quantifying those losses using the homoarginine technique, recently developed in this laboratory. Pigs fitted with permanent ileal T-cannulas were fed test meals containing homoarginine-labeled protein. The meals contained casein and increasing doses of trypsin inhibitors (Experiment 1) or alternatively either heat-treated or raw ground soybeans (Experiment 2). Following a casein meal (425 mmol nitrogen, no trypsin inhibitors), ileal protein was predominantly of endogenous rather than of exogenous origin (105 vs. 9 mmol nitrogen). Addition of isolated trypsin inhibitors (3000 mg) enhanced appearance of both endogenous and exogenous protein at the ileum (by 73 and 9 mmol nitrogen, respectively). Feeding raw instead of heat-treated soybeans in one single test meal caused a significant increase of endogenous protein from 217 ± 42 to 263 ± 47 mmol (mean ± SEM) and of exogenous protein from 16 ± 3 to 48 ± 14 mmol. If fed continuously for 1 wk, a raw soybean diet caused endogenous protein loss to rise significantly from 221 ± 26 to 432 ± 85 mmol. We conclude that ingestion of food containing trypsin inhibitor affects nitrogen balance more by losses of amino acids of endogenous secreta than by losses of dietary amino acids.

KEY WORDS: • protein absorption • homoarginine • trypsin inhibitor • soybean protein • pigs

¹ Part of this work was presented at a meeting of the Gesellschaft für Ernährungsphysiologie in Göttingen, Germany, March 31, 1992.

² Supported by the Deutsche Forschungsgemeinschaft Grant Ha 456/2-1 - II B 8 -

³ The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

⁴ To whom correspondence should be addressed at: Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, FB Ernährungsphysiologie "Oskar Kellner," J.-v.-Liebig-Weg 2, D-18059 Rostock, Germany.

Manuscript received 11 March 1993. Initial review completed 21 May 1993. Revision accepted 14 July 1993.

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