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Biotin and Biotin Analogs in Human Urine: Biotin Accounts for only Half of the Total^{1, 2,}

Department of Pediatrics, Division of Digestive, Endocrine, Genetic and Nutritional Disorders, University of Arkansas for Medical Science/Arkansas Children's Hospital, Little Rock, AR 72202-3591 ^* Department of Microbiology, University of Iowa College of Medicine, Iowa City, IA 52242

In studies using avidin-binding assays to measure the urinary excretion of biotin, biotin is sometimes assumed to be equal to the detectable avidinbinding substances present. High performance liquid chromatography was used to separate avidin-binding substances in human urine, and the chromatographic fractions were assayed for avidin-binding substances (biotin and biotin analogs) by a sensitive, specific assay based on binding of biotin to [¹²⁵I]avidin. In a study of ten normal adults, substantial amounts of avidin-binding substances other than biotin were detected, two of which were bisnorbiotin and biotin sulfoxide. These biotin analogs were initially identified by their chromatographic properties, and identities were confirmed by chemical conversion. The presence of avidin-binding substances in addition to biotin may have confounded previous measurements of the urinary excretion of biotin using avidin-binding assays. Because bioassay methods for biotin often use organisms for which one or more of these biotin analogs are growth factors, measurements of biotin in urine using some bioassay methods are likely to overestimate the concentrations of biotin.

KEY WORDS: • biotin • biotin analogs • urine • humans

¹ Supported by grants from the National Institutes of Health to D.M.M. (DK 36823).

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ To whom correspondence and reprint requests should be addressed at: Department of Pediatrics, Arkansas Children's Hospital, 800 Marshall Street, Little Rock, AR 72202-3591.

Manuscript received 18 March 1993. Revision accepted 14 June 1993.

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