QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Allen, C. B. Articles by Saari, J. T. Search for Related Content PubMed PubMed Citation Articles by Allen, C. B. Articles by Saari, J. T.

Isolated Hearts from Copper-Deficient Rats Exhibit Improved Postischemic Contractile Performance^{1, 2,}

United States Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58202

Dietary copper deficiency affects a number of enzymes, the function of which may influence the outcome of myocardial ischemia-reperfusion injury. Male weanling rats were fed diets that were adequate (>5 mg/kg) or deficient (<1 mg/kg) in copper. After 4 wk, the rats' hearts were isolated and used to study the effects of ischemia-reperfusion on intraventricular developed pressure (DevP), positive and negative rates of intraventricular pressure change (+dp/dt and -dp/dt) and release of lactate dehydrogenase and creatine kinase from the heart. The ischemia-perfusion protocol included a 15-min equilibration period, 30 min of warm, total ischemia and reperfusion for 30 min. Preischemic hearts from copper-deficient rats produced lower DevP than hearts from copper-adequate rats at all levels of preload. However, postischemic recovery of DevP was significantly greater in the hearts of the copper-deficient group. Furthermore, the postischemic patterns of lactate dehydrogenase and creatine kinase release in the two groups were significantly different. These findings indicate that, although dietary copper deficiency adversely affects a number of enzymatic systems, the functional recovery of hearts subjected to ischemia-reperfusion injury is improved when the diet is restricted in copper.

KEY WORDS: • heart • ischemia-reperfusion • copper • rats • free radical

¹ Presented in part at Experimental Biology 93, March 1993, New Orleans, LA [Allen, C. B. & Saari, J. T. (1993) Isolated hearts from copper-deficient rats exhibit improved postischemic contractile performance. FASEB J. 7: A103 (abs. 589)].

² The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.

³ Current address: Department of Nutrition, University of California—Davis, Davis, CA 95616.

⁴ To whom correspondence should be addressed: USDA, ARS, Grand Forks Human Nutrition Research Center, Box 9034, Grand Forks, ND 58202.

Manuscript received 8 February 1993. Revision accepted 1 July 1993.

This article has been cited by other articles:

				F. Dong, L. B. Esberg, Z. K. Roughead, J. Ren, and J. T. Saari Increased contractility of cardiomyocytes from copper-deficient rats is associated with upregulation of cardiac IGF-I receptor Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H78 - H84. [Abstract] [Full Text] [PDF]