QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Reicks, M. M. Articles by Hathcock, J. N. Search for Related Content PubMed PubMed Citation Articles by Reicks, M. M. Articles by Hathcock, J. N.

Prolonged Acetaminophen Ingestion by Mice Fed a Methionine-Limited Diet does not Affect Iron-Induced Liver Lipid Peroxidation or S-Adenosylmethionine

Food and Drug Administration, Washington, DC 20204 ^* National Center for Toxicological Research, Jefferson, AR 72079

This study determined whether acetaminophen (ACAP)-induced glutathione depletion was associated with liver lipid peroxide formation, or the concentrations of liver S-adenosylmethionine and S-adenosylhomocysteine in mice fed diets with L-methionine below or at the requirement level (0.25 or 0.5%) for 7 wk. Iron dextran (281 mg/kg body wt) or saline was administered for 2 d before measurement of lipid peroxide formation. Chronic dietary ACAP (0.5%) in mice fed 0.25% methionine caused a failure to maintain body weight even though food intake was similar to intake by all other treatment groups. Liver GSH (measured as nonprotein sulfhydryl concentration) and cysteine concentrations were depleted by ACAP and by ACAP plus iron. Liver lipid peroxide formation was increased by iron but was not altered additionally by ACAP ingestion. Liver glutathione peroxidase activity was increased by methionine in controls, whereas glutathione S-transferase activity was increased by ACAP ingestion in mice fed 0.5% methionine compared with controls. Liver S-adenosylmethionine and nuclear 5-methyldeoxycytidine concentrations were not affected by dietary ACAP or methionine. Liver S-adenosylhomocysteine levels were lower in mice fed ACAP and 0.25% methionine compared with mice fed ACAP and 0.5% methionine. In conclusion, chronic ACAP did not increase the susceptibility of mice to liver lipid peroxidation or alter the availability of methyl groups for methylation reactions.

KEY WORDS: • acetaminophen • S-adenosylmethionine • lipid peroxidation • glutathione • mice

¹ To whom correspondence should be addressed, at the current address: University of Minnesota, 162 Food Science and Nutrition, St. Paul, MN 55108.

Manuscript received 5 August 1991. Revision accepted 31 March 1992.