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Dietary Selenium Stabilizes Glutathione Peroxidase mRNA in Rat Liver^1,2,

Department of Food Science and Nutrition, Brigham Young University, 475 WIDB, Provo, UT 84602

The objective of these studies was to determine the step at which dietary selenium (Se) regulates the pre-translational expression of the gene for cytosolic Se-glutathione peroxidase (Se-GPX) in rat liver. Weanling male Sprague-Dawley rats were fed a Torula yeast-based Se-deficient diet, or the same diet supplemented with 0.5 mg/kg as Na₂SeO₃, for at least 40 d. Livers were excised and divided into three portions for isolation of nuclei, for purification of total RNA and for assay of Se-GPX activity. Nuclei were used in run-on transcription assays and for isolation of total nuclear RNA. Nuclear RNA and total liver RNA were probed with segments from a rat Se-GPX cDNA in Northern blot and slot blot assays. Despite marked differences in Se-GPX activity, there were no significant differences between dietary groups in the transcription rates of the Se-GPX gene. Species hybridizing to Se-GPX were not detected in nuclear RNA. However, steady state levels of Se-GPX mRNA were markedly reduced by Se deficiency. These results suggest that dietary Se exerts its effect on pretranslational Se-GPX gene expression at the level of cytosolic mRNA stabilization.

KEY WORDS: • selenium • glutathione peroxidase • gene expression • rats • liver

¹ Supported by NIH Grant no. DK41647 to M.J.C.

² A report of these results was made at the Annual Meeting of the Federation of American Societies for Experimental Biology, April 21–25, 1991, Atlanta, GA [Christensen, M. J. & Burgener, K. W. (1991) Effect of selenium deficiency on gene transcription. FASEB J. 5: A579 (abs.)].

Manuscript received 7 October 1991. Revision accepted 14 April 1992.

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