QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, S.-C. Articles by Fritsche, K. L. Search for Related Content PubMed PubMed Citation Articles by Huang, S.-C. Articles by Fritsche, K. L.

Dietary Fat Influences Ia Antigen Expression and Immune Cell Populations in the Murine Peritoneum and Spleen^1,2,

Department of Animal Sciences ^* Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO 65211

Peritoneal cells (PEC) and splenocytes were obtained from Listeria monocytogene (LM)-infected or noninfected mice fed a 20% fat diet rich in either (n-3) polyunsaturated fatty acids [(n-3) PUFA diet], linoleate [(n-6) PUFA diet], oleate (MONO diet), or saturated fatty acids (SAT diet) for 6 wk and were assessed for T cells, B cells, macrophages and Ia expression by flow cytometric analysis. In the peritoneum of noninfected mice, dietary fat did not affect total cell yield or the percentage of B cells, macrophages or Ia⁺ cells, but the (n-3) PUFA-fed group had a greater percentage of T cells than did the other groups. Among the LM-infected mice, the (n-3) PUFA-fed group generally had the highest percentage of B cells and the lowest percentages of T cells, macrophages and Ia⁺ cells in the peritoneum. Listeria monocytogene infection elevated peritoneal T cell numbers in all mice except the (n-3) PUFA-fed group. The density of Ia molecules on PEC was 40% lower in mice fed the (n-3) PUFA diet. In the spleen, dietary fat also influenced the immune cell populations and Ia⁺ cells. Two-color staining of spleen cells revealed that Ia⁺ splenocytes were predominately B cells. These data demonstrate that dietary fats influence Ia expression and immune cell populations and that the effects observed in one immune tissue or cell type may not be readily extrapolated to others.

KEY WORDS: • dietary fat • fish oil • mice • la antigen • immune cell populations

¹ Financial support for this research was provided by the Food for the 21st Century Program and the University of Missouri Agriculture Experiment Station.

² Contribution from the Missouri Agriculture Experiment Station, Journal Series Number 11,545.

³ Current address: Oklahoma Medical Research Foundation, 825 N.E. 13th St., Oklahoma City, OK 73104.

⁴ To whom correspondence and reprint requests should be addressed.

Manuscript received 30 July 1991. Revision accepted 14 January 1992.

This article has been cited by other articles:

				S. R. Shaikh and M. Edidin Immunosuppressive effects of polyunsaturated fatty acids on antigen presentation by human leukocyte antigen class I molecules J. Lipid Res., January 1, 2007; 48(1): 127 - 138. [Abstract] [Full Text] [PDF]

				S. R. Shaikh and M. Edidin Polyunsaturated fatty acids, membrane organization, T cells, and antigen presentation Am. J. Clinical Nutrition, December 1, 2006; 84(6): 1277 - 1289. [Abstract] [Full Text] [PDF]