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Reexamination of Protein Requirements in Adult Male Humans by End-Product Measurements of Leucine and Lysine Metabolism^1,2,

Gordon A. Zello^*,,, Joseph Telch^*,,,, Ruth Clarke^*,, Ronald O. Ball and Paul B. Pencharz^*,,,,3

The^* Division of Clinical Nutrition The Research Institute, The Hospital for Sick Children the Department of Nutritional Sciences the Department of Paediatrics, University of Toronto, Toronto, ON, Canada M5G 1X8 Paediatrics, University of Toronto, Toronto, ON, Canada M5G 1X8

We examined leucine and lysine metabolism in adult humans (n = 8 and n = 5, respectively) to assess the current estimate of protein requirement. Each subject consumed a controlled isoenergetic liquid diet for three 10-d periods at three intakes of protein, 0.6, 0.8 and 1.0 g·kg^-1·d^-1, in random order. Measurements of flux and catabolism were determined in each subject in both the fed and postabsorptive states, with a primed, 4-h continuous infusion of L-[1-¹³C]leucine and L-[-¹⁵N]lysine. Fluxes of leucine and lysine were not affected by feeding state or protein intake. Adaptation to different protein intakes did not affect the oxidation of leucine and lysine in the postabsorptive state, but leucine oxidation was significantly higher during the fed period compared with the postabsorptive period at a protein intake of 1.0 g·kg^-1·d^-1 (P < 0.05) and tended to be greater (P = 0.06) at an intake of 0.8 g·kg^-1·d^-1. Nitrogen balance assessed over the last 72 h of each dietary period was positive for all subjects at protein intakes of 0.8 and 1.0 g·kg^-1·d^-1 of protein and, hence, was consistent with the leucine oxidation data. These data suggest that experiments conducted during the postabsorptive period are not appropriate for estimating protein requirements. Leucine oxidation data obtained during the fed state estimate a protein requirement for young men of between 0.6 and 0.8 g·kg^-1·d^-1.

KEY WORDS: • protein • requirement • amino acid oxidation • flux • humans

¹ Supported by a grant from the Medical Research Council of Canada (MT10321). G. A. Zello was the recipient of an Ontario Graduate Scholarship.

² Part of this work was presented previously in abstract form: Zello, G. A., Telch, J., Clarke, R., Ball, R. O. & Pencharz, P. B. (1991). Effect of feeding on leucine and lysine kinetics in humans consuming varying protein intakes. FASEB J. 5(4): A564 (abs.).

³ To whom correspondence and reprint requests should be addressed.

Manuscript received 10 July 1991. Revision accepted 5 November 1991.

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