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Short-Term Regulation of the Renal Vitamin D Receptor in Rats by 1,25-Dihydroxycholecalciferol Is Calcium Insensitive^1,2,

Department of Biochemistry, University of Wisconsin-Madison, College of Agricultural and Life Sciences, Madison, WI 53706

Acute regulation of the vitamin D receptor in kidney by 1,25-dihydroxycholecalciferol and dietary calcium was investigated using vitamin D-deficient rats. 1,25-Dihydroxycholecalciferol administered to normocalcemic, vitamin D-deficient rats increased the renal receptor level, whereas serum calcium and phosphorus concentrations remained nearly constant. In hypocalcemic, vitamin D-deficient rats, 1,25-dihydroxycholecalciferol caused a sharp response at 4 h, which remained elevated for the remaining 20 h. Serum calcium rose gradually over 24 h, whereas serum phosphorus remained fairly constant. When hypocalcemic, vitamin D-deficient rats were fed a high calcium diet following 14 h without food, the serum calcium concentration increased and serum phosphorus concentration decreased, whereas renal receptor levels were unchanged. These data indicate that 1,25-dihydroxycholecalciferol rapidly regulates the renal vitamin D receptor, independent of serum calcium and phosphorus, but requires an adequate serum calcium concentration to sustain a prolonged effect.

KEY WORDS: • vitamin D • recetor • rats • regulation • kidney

¹ Supported in part by a program project grant #DK-14881 from the National Institutes of Health and a fund of the Wisconsin Alumni Research Foundation.

² Reprints are not available.

³ To whom correspondence should be addressed.

Manuscript received 20 February 1992. Revision accepted 29 July 1992.