Journal of Nutrition

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Journal of Nutrition Vol. 121 No. 9 September 1991, pp. 1305-1310
Copyright © 1991 by American Society for Nutrition
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Modulation of Lipid Chylomicron-Synthesizing Enzymes in Rats by the Dietary (n-6):(n-3) Fatty Acid Ratio

Magali Chautan1, Monique Charbonnier, Jeannie Léonardi, Marc André, Huguette Lafont and Gilles Nalbone

Unité 130 de l'Institut National de la Santé de la Recherche Médicale, 13009 Marseille, France

The effect of diets with various (n-6):(n-3) polyunsaturated fatty acid (PUFA) ratios and a constant polyunsaturated:saturated fatty acid ratio on the basal activity of chylomicron lipid synthesizing enzymes was investigated in rat intestinal microsomes. Enzymes studied were: acyl-CoA:cholesterol acyltransferase (ACAT); acyl-CoA:lysophosphatidylcholine acyltransferase (ACLAT); acyl-CoA:2-monoacylglycerol acyltransferase (MGAT) and acyl-CoA:1,2-diacylglycerol acyltransferase (DGAT). Results showed that after a 4-wk feeding period, ACAT, ACLAT and DGAT basal activities were significantly enhanced by the dietary fatty acids of the (n-3) series, whereas MGAT activity was not affected. When the highest (n-6):(n-3) ratio (51.0) was compared with the lowest (0.8), the increase attained was about 58, 76 and 73% for ACAT, ACLAT and DGAT, respectively. Fatty acid composition of microsomal lipids was drastically altered by the diets because (n-3) PUFA replaced the (n-6) series as the dietary (n-6):(n-3) ratio was lowered. These compositional changes could explain the observed modification in the membrane-bound enzyme activities. We suggest that (n-3) PUFA ingestion leads to an enhanced velocity of chylomicron synthesis in rats.

KEY WORDS: • rats • intestine • chylomicron • flsh oil • enzymes

1 To whom correspondence should be addressed.

Manuscript received 10 September 1990. Revision accepted 1 March 1991.






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