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Dietary (n-6) Polyunsaturated Fatty Acids Affect ß-Adrenergic Receptor Binding and Adenylate Cyclase Activity in Pig Adipocyte Plasma Membrane¹

Laboratoire de Nutrition et Sécurité Alimentaire, INRA-CRJ, Domaine de Vilvert 78352 Jouy-en-Josas Cedex, France ^* Service de Biochimie du Centre Hospitalier de Poissy et de la Faculté de Médecine Paris-Ouest, 78303 Poissy, France

The effect of dietary (n-6) polyunsaturated fatty acids (sunflower oil) on pig adipocyte ß-adrenoreceptor and adenylate cyclase activity was examined. Two adipose sites (subcutaneous and perirenal) were compared. The existence of two affinity classes for ß-adrenoreceptors was evidenced. Adenylate cyclase stimulation by isoproterenol was higher in the perirenal fat than in the subcutaneous fat, in parallel to a higher ß-adrenoreceptor density. When sunflower oil was included in the diet, the adenylate cyclase response to ß-agonists was greater, particularly in perirenal fat, as was the affinity of the adrenoreceptors in their high affinity state. However, the number of ß-adrenoreceptors was lower, suggesting that these are spare receptors. Adenylate cyclase stimulation by 5'-guanylylimidophosphate also revealed site- and diet-specific variations indicating alterations at the Gs-protein level. The adenylate cyclase catalytic activity (reflected by the forskolin-stimulated response) in the control group was higher in the subcutaneous fat than in the perirenal fat. In the sunflower oil-fed pigs, the catalytic activity was greater in the perirenal fat relative to controls, leading to similar values in both adipose tissues of sunflower oil-fed pigs. This indicates that the cyclase catalytic subunit activity also depends on the anatomical site of the fat deposit and is influenced by the diet as well. Correlation between these changes in the adenylate cyclase system are discussed in relationship with alterations in the plasma membrane structure.

KEY WORDS: • linoleic acid • adenylate cyclase • ß-adrenergic receptor • pigs

¹ This study was partially supported by grants from the Institut National de la Recherche Agronomique (AIP-INRA No. 87-4613).

² To whom correspondence should be addressed.

Manuscript received 26 March 1990. Revision accepted 22 January 1991.

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