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The Absorption of Pteroylpolyglutamate and Intestinal Pteroylpolygammaglutamyl Hydrolase Activity in Zinc-Deficient Rats¹

Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL 35294 ^* Department of Nutrition, University of California, Davis, CA 95616

The effect of dietary zinc deficiency on pteroylpolygammaglutamyl hydrolase (folate hydrolase) activity and on pteroylpolyglutamate absorption was studied in rats. Three groups of male Sprague-Dawley rats (zinc-deficient, restricted-fed and ad libitum-fed controls) were fed a semipurified 25% egg white protein diet. The zinc-deficient group received 0.7 mg zinc/kg diet, whereas restricted-fed and ad libitum-fed control groups received 106 mg zinc/kg diet. After 6 wk of feeding, intestinal mucosal folate hydrolase activity was determined, and the absorption of pteryl-U[¹⁴C]glutamyl-hexaglutamic acid ([¹⁴C]PteGlu₇) and [³H]pteroylglutamic acid ([³H]PteGlu) was measured after intragastric administration. The intestinal mucosal folate hydrolase activity of zinc-deficient rats was not significantly reduced compared with two control groups. No significant differences in the absorption of [¹⁴C]PteGlu₇ and [³H]PteGlu were found among the three groups. These results indicate that intestinal folate hydrolase is not zinc dependent in rats and the intestinal absorption of pteroylpolyglutamate is not reduced in zinc-deficient rats.

KEY WORDS: • zinc • zinc deficiency • rats • folate absorption • folate hydrolase • pteroylpolyglutamate

¹ Supported in part by National Institutes of Health grant 5P01 CA 28103.

² To whom correspondence should be addressed.

Manuscript received 27 July 1990. Revision accepted 1 November 1990.

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