QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mildner, A. M. Articles by Clarke, S. D. Search for Related Content PubMed PubMed Citation Articles by Mildner, A. M. Articles by Clarke, S. D.

Porcine Fatty Acid Synthase: Cloning of a Complementary DNA, Tissue Distribution of Its mRNA and Suppression of Expression by Somatotropin and Dietary Protein

Unit of Performance Enhancement, The Upjohn Company, Kalamazoo, MI 49001

A cDNA for porcine fatty acid synthase was isolated and used to examine the tissue distribution of fatty acid synthase mRNA within the pig and to determine the impact of recombinant porcine somatotropin (rpSt) and the level of dietary protein on fatty acid synthase mRNA abundance in pig liver and adipose tissue. A 1.5-kb cDNA representing the thioesterase domain of porcine fatty acid synthase was isolated from a lambda gt 11 liver cDNA library. Northern analysis with total RNA extracted from adipose tissue, liver, heart, lung, kidney and intestine revealed a single major fatty acid synthase mRNA species of 8–9 kb. The amount of fatty acid synthase mRNA in hepatic tissue was 25% of the amount in adipose tissue, which suggests that the liver may be a significant site of fatty acid synthesis in the pig. Fatty acid synthase mRNA abundance was significantly reduced in the adipose tissue (P < 0.01) and the liver (P < 0.1) by chronic daily administration (60 µg/kg) of rpSt. In addition, increasing the amount of dietary protein decreased (P < 0.1) the abundance of fatty acid synthase mRNA in adipose tissue but had no effect on liver fatty acid synthase expression. In contrast, the abundance of adipose fatty acid binding protein mRNA was unaffected by rpSt or dietary protein. These data indicate that the reduction in the level of fatty acid synthase mRNA is a factor in the pSt-mediated suppression of fatty acid synthesis in porcine adipose tissue.

KEY WORDS: • fatty acid synthase • pigs • gene expression • adipose • somatotropin

¹ To whom correspondence should be addressed.

Manuscript received 20 April 1990. Revision accepted 2 November 1990.

This article has been cited by other articles:

				T. E. Weber, B. T. Richert, M. A. Belury, Y. Gu, K. Enright, and A. P. Schinckel Evaluation of the effects of dietary fat, conjugated linoleic acid, and ractopamine on growth performance, pork quality, and fatty acid profiles in genetically lean gilts J Anim Sci, March 1, 2006; 84(3): 720 - 732. [Abstract] [Full Text] [PDF]